A page gel , stained by the petiodic acid - schiff ' s method to reveal glycoproteins , further displayed that the bindng - protein was a glycoprotein belonging to lectin , which contained 17 . 4 % ( w / w ) neutral carbohydrate content of the glycoprotein detected by the phenol / h2so4 method . peptide mapping was comparable to the reported protein in protein bank . the database homology search ( ncbi blast ) indicated that the binding - protein shared 70 - 80 % homogeneity to l - aspartate oxidase , aspartyl / glutamyl - trna ( asn / gln ) amidotransferase subunit b , glutamyl - trna reductase , histidyl - trna synthetase 连续梯度聚丙烯耽胺凝胶电泳、 sds一聚丙烯酞胺凝胶电泳和等电聚焦的结果表明该蛋白分子量为1 “ . skda ，由二个相同的亚基组成，亚基分子量为” . ikda ，等电点为8 . 25 .糖蛋白染色结合考马斯亮蓝染色的结果证实此结合蛋白是个糖蛋白，其中糖含量为17 . 44 % ，蛋白含量为82 . 56 % .凝集反应确定该糖蛋白也是一个凝集素
Dna fragments containing rst - 0x1 and rst - 0x2 as well as their promoters were abtained by pcr . the results of sequencing and blast analysis suggested these fragments shares 99 % identity with sinorhizobium meliloti 1021 . function of rst - oxl was unknown , while rst - 0x2 shares 81 % identity with gshb gene of rhizobium tropici , which encoding glutathione synthetase 序列测定和blast分析表明，它们和苜蓿中华根瘤菌1021的相应基因有99同源性， 042bm的rst - 0x1基因功能未知，而rst - 0x2基因和热带根瘤菌谷胱甘肽合成酶基因gshb有81同源性。
