Then using ecbp21 antibody and immunogold transmission electron microscopy method , we studied the subcellular localization of ecbp21 . the results indicated that the gold particles were mainly localized in the cell wall in callus cells and rachis cells of angelica dahurica . these results indicated that ecbp21 mainly localized in cell wall , which provide a direct evidence of the extracellular existence of ecbp21 . furthermore , using ecbp21 antibody and immunohistochemical method , we studied the organic specially distribution of ecbp21 , the results indicated that ecbp21 distributed in all organize , but it distributed more in leave n flower rachis than in leafstalk and root 首先，构建了ecbp21表达载体，诱导了重组蛋白的表达，并通过胶回收法获得了大量纯化重组ecbp21蛋白，制备了高效价、高特异性抗体；随后，利用ecbp21抗体，结合免疫胶体金电镜定位技术进行了ecbp21亚细胞定位研究，结果显示：在白芷愈伤组织细胞和花序轴细胞中金颗粒主要分布在细胞壁区域，而在细胞内未发现或仅有少量金颗粒分布，表明ecbp21蛋白主要定位于细胞壁区域，这为细胞外cambp ( ecbp21 )的胞外存在提供了直接证据：进一步，利用ecbp21抗体，通过免疫组织化学分析研究了ecbp21组织特异性分布状况，结果表明ecbp21在白芷各组织中均有分布，但在叶、花、花序轴中分布较多，而在叶柄、根中分布较少。
It is mainly characterized in the triangular - lanceolate leaves which are green but white with dark green reticulation along the midrib and more or less truncate at base ; the flowers are erect on the rachis and half - opened ; the lip is tripartite , glabrous , more or less urceolate in side view ; the hypochile is saccate , ovoid and big while the epichile is relatively tiny , reflexed , undivided and transversely oblong or subreniform 本种之主要特征为： ?呈三角?披针形， ?面?色，沿中?有白色斑块， ?基多少呈截形；花直?于花轴，半张；唇瓣呈三段? ，平?无毛，侧视时多少呈壶型；基段片囊? ， ?形，巨大，先段片相对微小、反卷， ?再细?而呈横向椭圆形。
The feathers attached to the arms and the tail evidently possess the structures of " modern feathers " , which consisting of the central shafts ( rachis ) and parallel barbs . dromaeosaurids are unusual group of maniraptoran theropods . they share many similar or the same features with archaeopteryx or other primitive birds , such as : u - shaped furcula , uncinate process , laterally facing glenoid of shoulder girdle , comparatively long forelimb , large semilunate carple , retroverted pubis , ascending process of astragalus , and " modern feathers " with rachis and barbs etc . these facts indicate not only that dromaeosaurids had developed many bird - like characters in course of the advanced maniraptorans toward birds , but also that there seemed to be the co - evolutionary trend between the feathers and the skeletal bones 奔龙类是一类较为特殊的手盗龙类( maniraptora ) ，它具有许多与始祖鸟archaeopteryx甚至其它早期鸟类相似或接近的特征，主要表现在：叉骨u形与始祖鸟和孔子鸟类的相同，背肋钩状突在孔子鸟类和个别反鸟类中也存在，肩臼窝绝大部分指向侧方类似于始祖鸟，肩胛骨与乌喙骨间的夹角非常接近90也和始祖鸟的相似，前肢从比例上讲显得较长，大的半月形腕骨使肘部能向侧方折过来，耻骨伸向后下方，距骨上升突与原始鸟类中的为同源结构，羽毛已有显著的分化且具备了现代羽毛的基本结构等等。
