Tnfa antagonists including mab against tnfa and tnf receptor ( tnfr ) , soluble tnfa receptor ( stnfr ) , mutant tnfa have been applied or tried to apply in the clinical with some positive therapy effects , but there were still some problems to limit the application of these agents 重组tnf及其拮抗剂(包括抗tnf单抗、抗tnf受体单抗、可溶性tnf受体、 tnf突变体)均已应用于临床并取得了一定的治疗效果。
For any other son to have stayed with his mother for four days at tr port , it would have been a condescension or a martyrdom , while i return , more contented , more peaceful - shall i say more poetic ! - than if i had taken queen mab or titania as my companion . 要是别的当儿子的陪他的母亲到的黎港去住四天,他肯定会觉得枯燥,厌烦,但我陪了她四天,却比陪伴玛琵仙后民间传说中的仙女,莎士比亚戏剧罗密欧与朱丽叶中有详细描写。
All the clones could react with swine antisera against prrsv and mouse antisera to prrsv , suggesting aa50 ~ aa55 domain on n protein might be the antigenic site recognized by mab ge3 . 4 . the gene fragment encoding the epitope recognized by mab ge3 was cloned into the expression vector pgex - 4t - 2 用prrsv阳性血清和鼠源抗prrsv抗体采用间接elisa进行鉴定,结果表明筛选到的噬菌体克隆可以与血清发生特异性反应,从而初步确定了单克隆抗体ge3所识别的抗原表位。
Studies of genes related to heart development in drosophila contribute to reveal the mechanisms of human heart development and the congenital heart diseases . to clone and identify new genes that control the heart development , by a way of chemical mutagen , ems , we have established 1 , 200 balanced - lethal lines on chromosome 2 and 3 . with the screening the 330 stocks with immunochemical method using heart - specific antibody , mab . no . 3 , we detected 60 lethal lines showing heart mutant phynotype 为了克隆和鉴定控制心脏发育的新基因,本研究利用化学诱变剂甲磺酸乙酯大规模地诱变果蝇,并且建立了1200个第二和第三染色体的平衡致死系,利用心脏组织特异抗体mab . no . 3对其中330个品系进行免疫化学方法筛选,观察到有60个致死系表现出心脏突变表型, 20个品系的心脏突变表型有待进一步证实。
24 , 48 , 72 hours later after transfection , we testified the expression of pp38 with mab h19 , and pp24 with the antiserurn of pp24 expressed in e . coli . the tests justified the expression of pp24 in prokaryotic and eukaryotic expressing systems . in order to study the correlation of pp38 and pp24 , we cloned pp38 gene and pp24 gene into pbudce4 为研究pp38和pp24之间的关系,将型mdvmd11株的pp38基因和pp24基因的完整orf克隆到真核双表达载体pbudce4 . 1中,转染cef后,通过ifa检测和用抗pp24多克隆血清进行western - blotting试验检测到了pp38和pp24磷蛋白的共表达。
Through three rounds of screening , seventeen clones were selected and used in competitive test . the mab ge3 could specifically inhibit eight out of seventeen clones from binding to swine antisera . based on the amin acid sequences deduced from the foreign sequence inserted in the phage , it was indicated that all clones shared the core sequence - p / ekphf , that was similar to aa50 ~ aa55 domain of n protein of prrsv 从第三轮亲和筛选的噬菌体中随机挑取17个克隆进行功能鉴定,结果表明8个克隆与mabge3具有较强的特异性结合力并可以被prrsv阳性血清阻断,测序发现7个克隆具有核心序列: p ekphf ,该序列与prrsvn蛋白aa50 aa55 ( p ekphf )具有较高的同源性。
Western blot analysis showed that rhpk - 5 ( recombinant human plasminogen kringle 5 , rhpk - 5 ) protein was recognized by mab same as native hpk - 5 . the result suggested that we obtained correct gene sequence of hpk - 5 and got the purified rhpk - 5 . section ii : construction of pbv220 / hpk - 5 vector for obtaining high - level hpk - 5 expression system , the hpk - 5 gene was recombined with plasmid pbv220 to construct the vector of pbv / hpk - 5 Coli )作为宿主,经sds - page分析,筛选表达量最高的菌株作为发酵用工程菌株;用western - blot方法鉴定hpk - 5因子的免疫学活性;用摇瓶发酵的方法,研究发酵培养基的体积(溶解氧) 、组成成份及诱导起始时间和诱导持续时间对目的蛋白表达量的影响,优化hpk - 5基因工程菌的表达条件。
By using western - blot , the fusion protein could not be react with antiserum to prrsv , whereas it presented a reactivity with swine antisera against prrsv and mab ge3 by elisa the results implied that the epitope might be one conformational epitope that was mainly composed of aa50 ~ aa55 domain on n protein Western - blot分析表明表达产物与prrsv阳性血清没有反应性,而elisa分析表明表达产物可与prrsv阳性血清和单克隆抗体发生反应。由此说明单克隆抗体ge3所识别的抗原表位可能是存在于n蛋白上以kphf为核心的构象型表位。
Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2 . construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr . the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e . co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii ) Mg _ 7重组噬菌体抗体库的构建及鉴定从培养的mg _ 7杂交瘤细胞中提取并分离mrna ,反转录成cdna ;利用pcr分别扩增mg _ 7单抗的重链及轻链可变区基因,并通过? dna连接子将二者连接起来形成mg _ 7单链抗体基因;将mg _ 7单链抗体基因插入pcantab5e ;将连接产物转化感受态tg1大肠杆菌,制备细菌形式的mg _ 7重组噬菌体抗体库;通过菌落计数和限制性酶切分析( ecor和hind )评估mg _ 7重组噬菌体抗体库的容量和重组率。