Lysozyme chloride powder has a wide area of application , such as food preserving ( cheese , low degree wine ) , pharmaceutical industry ( tablets , capsules , eyedrops , throat lozenges ) , daily chemicals ( tooth paste , cosmetics ) , baby food ( milk powder ) and scientific research 蛋清溶菌酶在食品保鲜（特别是奶酪，清酒生产） ，医药（药片，胶囊，眼药水，润喉液） ，日化（牙膏，化妆品） ，婴儿食品（母乳化奶粉）及科学研究中有着广泛的应用。
Using lysozyme as a model , the effect of static magnetic field on me activity of lysozyme was studied using information provided by impedance response , two response models were first time proposed , which contained the parameters of magnetic field intensity and the magnetization time of lysozyme , respectively 利用阻抗响应曲线提供的信息，以溶菌酶为代表酶，研究了静磁场对酶活性的影响。首次提出了包含磁化时间和磁场强度变量的响应模型。
Abstract : the article introduces the application of cold sterilization , super high pressuer sterilization , sterilization system of hydrogen peroxide - peroxidase - glucose oxidase , uht , htst , radioactive sterilization and lysozyme sterilization to the production of tea beverage ， and predicts their future prospect 文摘:介绍了冷灭菌、超高压杀菌、过氧化氢-过氧化氢酶-葡萄糖氧化酶体系杀菌、超高温杀菌、高温瞬时杀菌、辐照杀菌和溶菌酶杀菌等技术在茶饮料生产上的应用，展望了其应用前景。
The article introduces the application of cold sterilization , super high pressuer sterilization , sterilization system of hydrogen peroxide - peroxidase - glucose oxidase , uht , htst , radioactive sterilization and lysozyme sterilization to the production of tea beverage ， and predicts their future prospect 介绍了冷灭菌、超高压杀菌、过氧化氢-过氧化氢酶-葡萄糖氧化酶体系杀菌、超高温杀菌、高温瞬时杀菌、辐照杀菌和溶菌酶杀菌等技术在茶饮料生产上的应用，展望了其应用前景。
First , after investigation of two original strains " biological characteristics , we studied the main influence factors on protoplasts formation and regeneration in s . mycarofaciens and s . erythreus , and determined the best protoplasts formation and regeneration conditions of two original strains . the former shake - cultured in s " medium at 28 ? , 220r . min ~ ( - 1 ) for 24h , lysised by 3mg / ml lysozyme , keeping warm at 32 ? for 50 ~ 60min , regenerated on r _ ( 5 " ) medium , 28 ? for 5 ~ 6d . the latter used two - step culture , then used img / ml lysozyme keeping warm at 37 ? for ih ; the protoplasts were plated on r5 " regeneration medium at 28 ? for 5d 首先在对两亲株的生物学特性进行了鉴定后，考察了影响两亲株原生质体形成和再生的主要因素，确定了生米卡链霉菌和红霉素链霉菌原生质体形成及再生的最佳条件：前者用s培养基，在28 、 220r . min ~ ( - 1 )培养24h后，用3mg ml的溶菌酶在32恒温酶解50 60min ，得到的原生质体在干燥的r5培养基上28倒置培养5 6天，可得到再生率在20左右的再生菌落；后者采用二级菌丝培养，用1mg ml的溶菌酶在37恒温酶解1h左右，得到的原生质体也在干燥的r5培养基上28倒置培养5天，即可得到再生率在20左右的再生菌。
After careful studying their relative importance to immune response and the possibility of the match , seventeen sequences of interest were selected for further experiment , including estss analogous to 11 . 5kd antibacterial peptide , lysozyme , serine protease and its inhibitor , lectin , antifreeze protein , et al . primers designed according to the sequences were used to amplify the corresponding estss from both blood and cephalothorax cdna library 在仔细分析了它们在免疫系统中的重要性和在对虾中出现的可能性之后，从中选出了17条可能编码抗菌肽，溶菌酶，凝集素、丝氨酸蛋白酶及其抑制剂，抗冻蛋白等蛋白质的序列，以此为依据设计引物，在中国对虾的血液和头胸部cdna文库中扩增相应的序列。
By sequencing random - selected clones from the library and using bioinformatics , 54 novel expressed sequence tags ( ests ) were obtained , in which there were 14 full - length cdnas , including peroxiredoxin gene , hmg - box gene , ubiquitin gene , signal recognition particle , acetyltransferase gene and lysozyme gene 有14个克隆包含有完整的开放阅读框，其中包括抗氧化物酶基因prx 、 dna结合蛋白基因、泛素基因、信号识别蛋白基因、溶菌酶基因和乙酰基转移酶基因等具有重要生物学功能的基因。
We know that the mouth is glands , they kept secreted in saliva , especially in the daytime by chewing , speaking of oral action , functional active glands , saliva secretion of large amount , which is in addition to saliva digestive function , due to its inhibition contain a lot of bacteria lysozyme , oral a self - cleaning and scouring role , which is very important oral self - protection 我们知道，口腔内有腺体，它们在不停地分泌唾液，尤其在白天通过咀嚼、说话等口腔动作，使腺体功能活跃，唾液的分泌量也大，这些唾液除具有消化功能外，由于其内含有大量抑制细菌的溶菌酶，对口腔有冲刷和自洁作用，这也是口腔很重要的自我保护作用。
The results of lauryl sodium sulfate - polyacrylamide gel electrophoreses ( sds - page ) of the aggregate precipitate and supernatant and the result of high - performance size - exclusion chromatography of the supernatant indicated that , by wrongly linked intermolecular disulfide bonds soluble bi - molecular and tri - molecular egg white lysozyme aggregate could be simultaneously formed except being renatured to native and active egg white lysozymes during the refolding procedure of denatured - reduced egg white lysozyme ; the aggregate precipitate could be further formed by the non - covalent bonds interaction between the soluble hi - molecular egg white lysozyme aggregates , and the soluble tri - molecular egg white lysozyme aggregate could still stay at the supernatant 沉淀和上清液的不连续十二烷基硫酸钠聚丙烯酰胺凝胶电泳( sds - page )和高效凝胶排阻层析分析结果表明，还原脲变性蛋白溶菌酶在稀释复性过程中除了能够复性成天然态蛋白溶菌酶分子外，还会形成可溶的蛋白溶菌酶分子二聚体和三聚体，二聚体和三聚体主要是靠分子间二硫键的错配连接而成的；可溶的蛋白溶菌酶分子二聚体之间通过非共价键相互作用而形成集聚体沉淀，而可溶的三聚体溶菌酶分子则仍处于复性液上清液中。
A web - based blast is also constructed to facilitate the process . and the contigs and ests sequences are formatted as subject database . by similarity search , about 442 estss were found possibly encoding immune related proteins , such as lysozyme , lectin , serine protease , serpin , thioredoxin , sod and so on 为了方便相似性比对，在由四台pc机并行组成的机群( pc - cluster )上将blast程序和interproscan程序本地化，并且将contig和ests序列文件做成blast程序中的序列库文件以供搜索。
