Ocular injury to icr mice from embryonated eggs of toxocara canis , which had been maintained in 2 % formalin for 14 months at 4 c , was evaluated by microscopic and pathologic assessments at 1 , 2 , 3 , 5 , 7 , 28 , 56 , 84 , 112 , 140 , 168 , 196 , 294 and 469 days post infection ( dpi ) 摘要本研究探讨将储存于2 %福马林液内，于4 c下保存14个月之犬蛔虫受孕卵感染小鼠第一天至第四百六十九天后，藉显微镜检视与病理学的研究评估孵化的幼虫对小鼠的眼部损伤情形。
The results showed that the feeder layers prepared by icr and kunming mice had no significant effect on adherence of embryos and proliferation of icms . the trypsin , which affected the isolation of es cells remarkedly , should be used with concentration of 0 . 125 % trypsin + 0 . 02 % edta for kmsmin 从实验结果来看， icr小鼠和昆明小鼠两种来源的饲养层细胞对胚胎贴壁、 icm增殖无影响，消化液作用影响明显，以0 . 125 trypsin + 0 . 02 edta消化10 15min为宜。
In the laboratory experiment part , human peripheral blood , cultured cells and icr mice were study objects . the changes of mitotic chromosome numbers were measured by human metaphase chromosome counts and statistic analyzed used x2 - test . the changes of meiotic chromosome numbers were measured by mice one - cell zygote chromosome counts and statistic analyzed usedx2 - test . the effects of low dose ionizing radiation on the expression of topoisomerase ii were measured by immunocytochemistry , western blot and rt - pcr 流行病学结果显示长期小剂量辐射接触与染色体不分离呈正相关，为进一步在细胞遗传学和分子生物学方面研究小剂量电离辐射与染色体不分离关系及其机制，本课题第二部分以外周血、培养细胞、 icr小鼠为研究对象，用外周血染色体计数和单细胞受精卵染色体计数的方法研究小剂量辐射和拓扑异构酶复旦大学2000级博士生学位论文11a抑制剂及其二者的协同效应对有丝分裂和减数分裂染色体不分离的影响，用免疫细胞化学染色、 westernblot 、 rt pcr等方法研究了电离辐射引起拓扑异构酶a表达变化。
Mouse blastocysts and morulas were cultured in rat heart conditioned medium ( rh - cm ) . as far as the following indexes attaching ratio , icm growth ratio and es clone formation ratio are concerned , the choice of blastocysts is much better than morulas ( p < 0 . 05 ) , therefore mouse blastocysts were more suitable than morulas to get es cells 源于icr小鼠icm的es细胞分离克隆研究试验以rh - cm为培养基培养处于不同发育阶段的小鼠胚胎，发现由囊胚分离的es细胞传代后es集落的出现率显著高于桑椹胚( p 0 . 05 ) ，故囊胚更适合作为es细胞分离克隆的材料。
We used the icr and kunming mice and got the embryos of 3 . 5dpc by means of superovulation , then cultured the embryos on the feeder layer derived from icr or kunming mice , dispersed the inner cell masses ( icms ) or es cell colony in the appropriate time . in this period , we analized the effects of feeder layer , trypsin and embryos isolated from uteri of different varietal mice on the es cell lines 以icr小鼠和昆明小鼠为实验对象，采用超数排卵的方法获得小鼠胚胎，培养在用丝裂霉素c处理过的icr小鼠和昆明小鼠mef细胞饲养层上，选取适当时间离散增殖的icm和类es细胞集落，分析了在小鼠es细胞建系过程中，饲养层细胞、消化液及不同品系来源的胚胎的影响。
Compared with control group , rbc - cabrr in hyperoxia group showed more significant increase and rbc - icr more significant decrease . , up to 24 hours later , even lower than pre - exercise level . it suggested that hyperoxia intake , to some extent , may accelerate the recovery rate of immunosuppression after acute exercise , and that hyperoxia intake can serve as a method to eliminate exercise - induced fatigue ? ? df ? ?主要反映在红细胞免疫功能的变化，吸氧组rbc c3brr在恢复30分钟时上升更明显，同时icr下降也更明显（吸氧组p队05 ，对照组p o0引，且恢复n小时下降至安静值以下。
Embryonic stem ( es ) cells were derived from the inner cell mass ( icm ) of preimplantation embryos , and embryonic germ ( eg ) cells from primordial germ cells ( pgc ) . both es and eg cells were cultured to determine the factors affecting on isolation , cloning and passage of pluripotent stem cells from icr mouse embryos 试验以icr小鼠胚胎内细胞团( icm )和原始生殖细胞( pgc )为材料，采用不同培养体系分离克隆胚胎多能干细胞，探讨icr品系小鼠胚胎多能干细胞分离克隆的基本方法，以及一些因素对其分离、培养、克隆、传代等效果的影响。
Pulmonary fibrosis was induced in the model group by intranasal instillation of bleomycin 5 mg kg . hxd were given via gastrogavage to the hxd prevention group and treatment group respectively at 48 h before and 14 days after they were modeled with the same method , and the groups of ligustrazin and prednisone , treatment and prevention , were managed the same as for hxd groups , but with ligustazin and prednisone instead of hxd respectively . bronchoalveolar lavage fluid balf and lung tissue of animals were gathered on day 28 cellular ultrastructural changes of lung was examined under electron microscope by he , masson stain ; content of hydroxyproline in lung tissue was assessed by acidolysis method ; and concentration of transforming growth factor 1 tgf 1 in balf was assessed by elisa 方法68周雄性icr小鼠分8组,模型组bm组经鼻滴入博莱霉素blm 5 mg kg建立肺间质纤维化模型,化纤汤预防治疗组pht组于滴入blm前48 h予化纤汤灌胃，化纤汤治疗组ht组于滴入blm后第14天用化纤汤灌胃,同时设立丹参预防治疗组pdst组及丹参治疗组dst组，地塞米松预防治疗组pdt组及地塞米松治疗组dt组，并与正常小鼠nc组进行对照。
20 students from scnu served as the subjects , and they were randomly divided into the hyperoxia group and the control group . the venous samples were taken before exercise , just after exercise , post - exercise somin and 24h , respectively . the immune parameters included rbc - csbrr , rbc - icr , immunoglobulins ( igg , iga and igm ) 将实验对象分为吸氧组和对照组，测得两组安静时、运动即刻、恢复30分钟和恢复24小时的免疫指标的变化，包括红细胞免疫功能指标，即红细胞补体受体花环率( rbc - c _ ( 3b ) rr )和红细胞免疫复合物花环率( rbc - icr ) ；免疫球蛋白igg 、 iga 、 igm ，并对上述指标的变化趋势作了分析。
