Arginine rich human histone h3 is a kind of basic nucleosome protein . in the medium of physiological condition , by electrostatic interaction , histone h3 which arginine impart to it a positive charge binds to dna which phosphate groups impart to it a negative charge . in this paper , in order to establish a new technology of transfection , the functional domains la and ii gene segments of pea were lined with histone h3 gene segment , then a recombinant fusion protein was constructed which serves as c arrier for transfer of dna via receptor - mediated endocytosis 本实验在已成功获得的pea功能区a ，功能区基因片段与人组蛋白h3基因片段的克隆菌株的基础上，构建原核表达载体，将克隆质粒pmd - 18t - pea经nco ， mlu双酶切，回收酶切产物；将克隆质粒pmd - 18t - h3经mlu ， xho双酶切，回收酶切产物。
The photoelectric property of a - si : h films is closely associated with hydrogen content in films . on the one hand , hydrogen incorporated as monohydride ( si - h ) saturates dangling bonds in films , and on the other hand , hydrogen incorporated as polyhydride ( si - h2 , si - h3 , ( si - h2 ) n ) introduces defect in films and thus increases the density of localized electronic states in band gap A - si ： h薄膜的光电特性同膜中的氢存在密切关系，一方面，氢以单氢化合物( si - h )方式结合到膜中，从而饱和了膜中的悬挂键；另一方面，氢以多氢化合物( si - h2 、 si - h3和( si - h2 ) n )方式结合到膜中，反而在膜中引入了缺陷，使带隙中的局域态密度增大。
