Methods subacute senile mouse model was established by injection of d - galactose . the splenic cells were isolated from mice and cultured in vitro following cona stimulation . expression of cd 137 on splenic t cells in the model and normal control groups were compared with each other by flow cytometry method at various cultural times after cona stimulation 方法取5周龄balb c雄性小鼠，颈背部皮下注射d -半乳糖，分别于造模型后一到五个月取脾脏淋巴细胞，同时取自然衰老小鼠脾脏淋巴细胞，在培养的第0 、 24 、 48 、 72 、 96 、 120 、 144h收集细胞，用流式细胞仪分析脾脏t细胞cd137的表达阳性率。
Conclusions ( 1 ) subacute senile mouse model can be used in immunosenesence reseach . ( 2 ) cd 137 may be an marker of aging . ( 3 ) the protection to aicd of cd137 decreased , indicate the function of cd137 was unusual . ( 4 ) the effect of d - galactose can modelling the different stage of aging . ( 5 ) the decrease of cd 137 expression on t cell from subacute senile mouse model and the aging mouse were due to the declining of their mrna . ( 6 ) the expression of cd 137 on t cells from from subacute senile mouse model and the aging mouse have a time dependently derease and the peak of cd 137 expression appeared earlier while the aging keep going on 结论( 1 ) d -半乳糖致亚急性衰老小鼠模型t细胞cd137分子表达变化规律与自然衰老小鼠相似，造模两个月后的大剂量组小鼠即可用于衰老个体cd137分子的相关研究。 ( 2 )模型小鼠cd137分子表达随衰老的发生发展呈规律性变化，提示该分子可能为t细胞衰老的分子标志。 （ 3 ）即使在衰老状态下，机体对cd137分于的表达依然具有调控作用，提示兔疫系统的代偿能力仍然存在。
Results : it was about genomic polymorphism analyses of 5 parts of brain ( brain regions ) , composed of cortex , hyppocampus , sriatum , hypothalamus and cerebellum , in variety of the sd - rats . 1 , it was showed that special genomic rapds were different between the sd - rats at ages of 3 months old , 6 - 8 months old , 18months old and 24 months old , and there was some correlation between sd - rat " ages of months old adding up and increase of brain regions with special rapds . 2 , special genomic rapds of the brain aging sd - rat at the age of 24 months old was the same as that of the brain aging sd - rat induced by - d - galactose from the age of 3months old 结果对不同生理状态( 3月龄， 6 - 8月龄， 18月龄， 24月龄)下的脑皮层、海马、纹状体、下丘脑、小脑等五脑区进行基因组dna多态性分析，发现：不同月龄大鼠脑区的基因组特异性rapd分子标记不同，月龄的增长和脑区基因组特异性rapd分子标记的增多呈一定的相关性；对不同实验处理( - d -半乳糖致脑衰老，花粉、复方氨基酸、吡拉西坦抗脑衰老)后的五脑区进行基因组dna多态性分析，发现： - d -半乳糖致脑衰老与24月龄自然脑衰老脑区的基因组特异性rapd分子标记相似，花粉、复方氨基酸、吡拉西坦抗均不同程地改变了- d -半乳糖致脑衰老脑区基因组特异性rapd分子标记。
