And there is a serious problem of iron deficiency in which plant food is the main food resource , especially in developing country or area . ferritin , widely distributing in plants , animals and microorganisms , is a key iron storage , protein constructed from 24 subunits 铁蛋白( ferritin )是广泛存在于动物、植物和微生物中的一种由24个亚基组成的贮铁关键蛋白质，其贮存的铁具有可溶、无毒和生物可给性等特点。
We currently study the following projects ： ( 1 ) the biomineralization of nano - magnetic particle such as magnetotactic bacterial and ferritin ; ( 2 ) the magnetic research of biongenic nano - magnetic particles ; ( 3 ) the influence of the geo - magnetic field to the life 我们现阶段主要研究以下课题： （ 1 ）趋磁细菌，铁蛋白等为代表的纳米磁性颗粒的生物矿化作用； （ 2 ）生物源纳米磁性颗粒的磁学研究； （ 3 ）地磁场对生命活动的影响。
The researches acquired suggest that manipulating ferritin expression and other soluble components of seed iron in soybeans and possibly other seeds , using mendelian genetic technique and biotechnological approaches , could contribute to a sustainable solution to the global problem of iron deficiency 已有的研究表明，使用孟德尔遗传方法和生物技术途径提高大豆和其它谷物类种子中铁蛋白的表达和其它可溶性铁成分，将有助于全球性缺铁问题的有效解决。
Under the conditions of sds - page , it depolymerized into only one subunit of 28 kd , which reveal that the subunit of purified ferritin of pea is undivided 4 . preparation of antiserum of pea ferritin the purified pea ferritin was mixed with freund ' s complete adjuvant or freund ' s incomplete adjuvant , and then administered through palmula , back , abdominal cavity , vena auricularis and other routes for rabbits . seven weeks later , the litre of antiserum reached 1 i 32 4 、免疫家兔制备豌豆铁蛋白抗血清民二功寻咬径b一仑一以纯化的豌豆铁蛋白作为抗原，并分别加入弗氏完全佐剂和弗氏不完全佐剂等兔疫家兔，经过足掌、背部皮下、腹腔和耳静脉等途径进行免疫， 7周后获得效价高达1 ： 32以上的豌豆铁蛋白抗血清。
In this paper , the pea ferritin was purified from pea seed , and its antiserum was raised from rabbits for investigating the expression of transgenic plant . the main experimental results were summarized as following : 1 . establishment of ferritin detection system 本论文开展了豌豆铁蛋白的纯化、免疫家兔制备豌豆铁蛋白抗血清以及植物铁蛋白免疫学检测等一系列的研究，现将主要研究内容和结果总结如下： 1 、铁蛋白检测系统的建立纯化过程中以邻菲咯啉显色法检测铁蛋白，具有简单、灵敏、直观等优点。
However , it is necessary to acquire the antibody or the antiserum , which could specially react with the expression protein of die objective gene transferred into the transgenic plant according to the characteristics of high homology and immune cross - reaction among plant ferritin , using the special immune serum of pea ferritin , the content of plant ferritin could be detected for studing the ferritin expression of transgenic plant by the technique of immunoassay such as immunoprecipitation , eljsa and western blotting 利用免疫检测技术进行植物转基因的表达检测是一种简单、灵敏、快速、可靠的方法，但其前提条件是要有与转基因植物目的基因表达的蛋白质发生特异性免疫反应的抗体或抗血清。根据植物铁蛋白之间有高度同源性和交叉免疫反应的特性，利用特异性的豌豆铁蛋白抗血清，就可通过免疫沉淀、 elisa或western杂交等免疫检测方法进行植物铁蛋白含量等的检测，从而更好地进行转基因方面的研究。
During the course of purification , the method of orthophenanthroline staining is used to detect the ferritin . 30 | il of the fractions eluted from the aca ^ gel column were pulled into the cells of the minim incubation plate . then sopil 20 % dithionite and 40 | il 0 . 1 % orthophenanthroline solution were pulled into respectively 在酶标板小孔中加入30 l待测液，加入30 l20的连二亚硫酸钠溶液还原后，再加入40 l0 . 1邻菲咯啉溶液，根据有无桔红色颜色产生确定是否含铁蛋白。
The crude extraction of dry pea seed , which was obtained through marination , homogenization , filtration , centrifugation and other methods , was precipitated by 50 mmol / l ( final concentration ) mgq2 . the pellet was chromatographed on aca ^ gel filtration and deae - cellulose 52 ( de 52 ) anion - exchanger . single eluting peak containing ferritin was obtained finally 2 、豌豆种子铁蛋白的纯化豌豆种子经浸泡、匀浆、过滤、离心等操作得到豌豆铁蛋白粗提液，再用终浓度为50mmol / l的mgcl _ 2盐析等处理后，经aca _ ( 22 )凝胶过滤柱层析和deae -纤维素52 ( de52 )阴离子交换柱层析等方法进行纯化，得到单一的豌豆铁蛋白洗脱峰。
