The numhers of day - 4 ebs per milliliter culture media are 44 . 83 1 . 22 ( n = 6 ) and 43 . 17 1 . 05 ( n = 6 ) respectively when cultured by hyclone fbs and homemade green season fbs 在含进口hyclone胎牛血清或国产四季青胎牛血清的培养液中培养， es d3细胞形成的4debs数量分另为44
The ebs maintained for a total of 2 - 3 days with a colony or individual appearance could be further differentiated into neural lineages , epithelium and blood vessel - like structure 当简单胚体贴壁继续培养，可出现不同类型的分化细胞，如神经样细胞，上皮样细胞等，有时可见血管样结构出现
Eg cells of the 2th and 4th passage were akp ( alkaline phosphate ) positive . when cultured on degenerated feeder layers or in suspension , eg c ells formed embryoid bodies ( ebs ) in vitro 当eg细胞脱离饲养层悬浮培养，或在衰老的饲养层上延迟培养时，发现eg细胞或单个存在，或聚集成团，形成类似于早期胚胎的囊状胚体结构
First , it is difficult to analyze and control each regulatory step of differentiation in this method because embryonic bodies ( ebs ) , forming in the process of induction , contain many different kinds of cells (二) upr途径分子在由ra诱导es细胞得到的神经细胞分化初步模型中作用的研究1 . rt一pcr方法检测upr途径分子irela的表达。
The numbers of day - 4 ebs per milliliter culture media are 47 . 33 l . 48 ( n = 6 ) when cultured in glass container and 310 . 83 2 . 92 ( n = 6 ) in petriperm bacterial - culture dish and there is no eb formation in cell - culture dish 001 ） 。在铺有粘附物质的细胞培养皿中培养， es干3细胞不形成ebs ；在玻璃培养瓶中培养， es d3细胞形成的4debs数量为47
Spontaneous differentiation of es cell d3 line in vitro es cell d3 line via suspension culture can congregate small mass within 24 h , and large numbers of aggregates called embryoid bodies ( ebs ) gradually formed after 2 to 3 days of culture 5 . es一d3细胞系的自主分化es一d3细胞悬浮培养， 24h后可聚集成小的细胞团，第2 ~ 3d时形成大量的类胚体( ebs ) 。
Embryonic stem ( es ) cell lines isolated from primitive embryonic cells are highly undifferentiated and totipotent cells . in vitro , es cells can spontaneously differentiate into embryoid bodies ( ebs ) containing derivatives of three germ layers 胚胎干细胞( embryonicstemcell ， escell )系是从早期胚胎细胞分离培养而建立的高度未分化的具有全能性的细胞系。
After being cultured three generations and depleted feeder cell layers , the es - d3 cells differentiated into ebs efficiently in the culture media supplemented with homemade green season fbs and - me in petriperm bacterial - culture dish 二、 inbmec cm诱导小鼠es细胞生成造血干祖细胞将获得的4debs制成单细胞悬液，加入inbmec cm诱导4debs的单细胞生成造血干祖细胞。
It mainly expatiates the daily examining schedule , monthly examining schedule , yearly examining schedule , new facility for use , schedule for running threshold requisition of relay protection , and then puts up the collectivity designing via hipo ( hierarchy plus input , process , output ) figure . interfaces is very important for any system . a system with no interfaces is lone . this paper discusses the interface scheme , lodging five ways : network programming , directly accessing the table of the data source database , text file , http , transmitting data through the serial interface of computer . at last this paper lodges the interface schemes of dmis aiming at the instance of chongqing power dispatch and communication central : network programming , text file , http with ems ( energy management system ) and directly accessing the table of the data source database , http with ebs ( energy business system ) 本文对接口方案进行论述，提出了五种接口方案：网络编程、直接访问数据源的数据库表、形成文本文件方式、超级链接方式、数据通过计算机串口进行转发，并针对重庆电力调度通信中心的具体情况提出了dmis的接口方法：与ems ( energymanagementsystem )的接口采用网络编程、形成文本文件方式、超级链接方式，与ebs ( energybusinesssystem )的接口采用直接访问数据源的数据库表、超级链接方式。
The ebs of different days gained by means of hanging , suspending and suspending following hanging were processed by all - trans retinoic acid ( ra ) for 4 days . during this period , we observed the differentiation process and compared the differentiation ratio of es cells processed by means of hanging , suspending for several days . based on this study , we induced the ebs by ra of different concentration in order to study the effect of ra on the differentiation of es cells into neuron - like cells 首先从胚体( ebs )本身探讨es细胞内部调控诱导分化的机制，用悬浮培养、悬滴培养、悬浮转悬滴培养的方法培养出不同时间的ebs ，再加以ra处理，观察比较ebs对es细胞神经分化的影响；在此基础上，用不同浓度的ra处理ebs ，分析诱导剂ra对es细胞神经分化的影响。
