- acetolactate decarboxylase are widely found among bacterial strains but not in other groups of organisms . the enzyme has been demonstrated to be effective for removal of acetolactate and widely used in beer product . in this paper , - acetolactate decarboxylase from bacillus subtilis was purifed to homogeneity from cell extract by ammonium sulfate - fractionation , heat treatment , deae - sepharose fast flow column chromatography 本文对来源于枯草芽孢杆菌( bacillussubtilis ) 3226 - 5的-乙酰乳酸脱羧酶经硫酸铵分级沉淀、热处理、 deae - sepharosefastflow离子交换柱层析等分离纯化步骤，得到sds - paeg电泳纯，通过n末端氨基酸序列分析验证酶蛋白的纯度。
