W7 was added when the cells entered anaphase , we found that although some cells had elongated , the distribution patternof cam at central spindle was normal , and it seemed that a cleavage furrow would possibly form soon , however , these cells stopped cytokinesis at this stage , no furrow ingression followed 在细胞进入有丝分裂后期时，加入w7 ，我们发现有的细胞虽已拉伸变长，中区纺锤体处也有gfp cam的分布，存在形成分裂沟的迹象，但分裂沟却不继续缢裂加深。
We also stained the embryos with anti - arp3 antibody and found that these two kinds of antigen are generally co - localized but arp3 were not detected in cleavage stage embryos . it was deduced that cortactin functions in the cytokinesis independent of arp3 . and microinjection of anti - cortactin antibodies into the i cell stage embryos leaded to fatal developmental defects because of the unequal cytokinesis 在利用抗ap3的抗体进行的胚胎整装染色中，激光扫描共聚焦显微镜观察发现， arp3在卵裂期没有表达， mbt后，在细胞皮层区检测到arp3蛋白的阳性染色，在随后的发育过程中arp3与皮层蛋白共分布。
In this paper we reported the engagement of cortactin in cell movements in vivo for the first time . we also found that , besides the traditional idea about the role it plays in cell motility , cortactin was also involved in cytokinesis and played undispensensable role in nleava stage independent of arp2 3 complex 这表明皮层蛋白除了传统意义上了解的参与细胞主动运动外，还在卵裂期发育事二且j中科院硕士学位论文喻丹皮层蛋白（ cortactin ）在斑马鱼早期胚胎发育中的分布图式和功能分析件中扮演重要角色。
3 . when the cells were treated with w7 , a ca2 + / cam inhibitor , the central spindle assembly was affected , and the abnormal central spindle was formed ; besides , when w7 treatment was applied at an early stage of cytokinesis , the division furrow index would decrease substantially . these results show that cam is really involved in the regulation of furrow formation and this involvement may be fulfilled by regulating the dynamic structure of central spindle 我们利用w7抑制cam活性，发现cam活性丧失会引起中区纺锤体结构异常；如用w7处理同步化于胞质分裂早期的细胞，可使细胞的分裂沟形成指数明显下降，这表明cam的确参与调控了分裂沟的形成，而且可能是通过调控中区纺锤体的动态结构参与这一过程的。
By using gfp - cam fusion protein , we have observed the detailed dynamic redistribution of cam in hela cells during cytokinesis . in tri - polar cell model , we have studied the relationship between the distribution pattern of cam and the formation of the cleavage furrow . furthermore , we have made the study on the regulation mechanism of cam during the whole process of cytokinesis by inhibiting its activity at different cytokinesis stages 我们采用绿色荧光蛋白( gfp )标记技术，重点观察了胞质分裂期hela细胞中gfp - cam的动态分布；应用三极细胞模型研究了cam的分布与分裂沟形成之间的关系；还通过在胞质分裂不同阶段抑制cam的活性研究了cam在整个胞质分裂过程中的作用机制。
