Within 2h isolation from the follicle 100 % of oocytes underwent germinal vesicle breakdown and both the nucleolus and the nuclear envelope disappeared . after culturing for 6 h , prometaphase occurred in 90 % oocytes 本研究旨在探索一种崭新的、化学试剂诱导去核卵母细胞为核受体的、无透明带的、手工体细胞核移植方法。
Three dimensions culture system is proved to be suitable for cultunng the follicles in diameter of 60 ~ 100 m , while the two dimension culture system is proved to be suitable for culturing the follicles in diameter of 100 - 140 m 结果发现，三维培养法适宜培养60 100 m的卵泡，而二维法有利于100 140 m卵泡的体外发育。
By analyzing the result of survey , we can find that din and nh3 in sea area of deep water net - cage culturing is more higher than other sea area , but the pollution is smaller 摘要通过对箬跳、青山2个深水网箱养殖区的水质状况的调查数据分析发现，养殖海区的无机氮和氨氮含量明显升高，但是深水网箱养殖海区的总体污染状况要比海区小。
But because of having not mastered the growth conditions of mycelia and not succeeded in culturing fruit bodies , it was retarded the pace of developing and utilizing the new medical source 但由于对其菌丝体的生长条件还没有完全掌握，子实体的栽培技术还未成功，致使对桑黄各方面的研究进展很慢，阻碍了这一新兴中药资源的开发进度。
Therefore , in order to settle a better terrace for children ' s healthy development , the educators have an unshirkable duty on developing the children ' s potency and culturing their high power of acting on their own 因此，如何加快幼教改革的步伐，有效地开发幼儿的潜能，培养幼儿高度的自主性，为幼儿将来的健康发展奠定良好的平台，是幼教工作者义不容辞的责任。
The development of theanine biosynthesis by microorganism fermentation and culturing cells of camellia sinensis was summarized in this paper and the possibility of theanine biosynthesis from the gene engineering strain was also discussed 摘要综述了近年来应用微生物发酵法、茶树细胞培养法生物合成茶氨酸的研究进展，讨论了构建基因工程菌生物合成茶氨酸的可行性。
The results of sds - page and western blot showed that the culture supernatant of transforments contained the correct glycosylated e2 protein and the recombinants could still secretedly express the specific proteins after culturing for 8 generations 经甲醇诱导表达后， sds - page和westernblot结果证明了p . pastoris培养上清液中含有正确糖基化的e2蛋白，表达量约250mg / l 。
With china ' s entry into the wto , the global economy system comes to be a whole organically . helping the enterprises of our country build competitive prevalence and culturing the ability of sustainable development is extremely urgent 随着我国加入世界贸易组织，全球经济一体化体系的逐步建立，帮助我国企业建立竞争优势，培养我国企业的可持续发展能力显得迫在眉睫。
After 15h of maturation . 98 % of oocytes released the first polar body and thus the first meiosis of mouse oocytes ended . secondly , strong chromosome to chromosome binding was induced by culturing pro - mi oocytes in demecolcine supplemented medium 第一次减数分裂期小鼠卵母细胞经化学去核后，去除透明带，并胎儿成纤维细胞粘合、电融合并srcl2激活，培养于微孔中。
