In order to find an explanation and to determine the possible mechanism of these biological effects , many in vitro and in vivo studies of emf effects have been published . these studies were involved in many enzymes ( such as odc , succinate dehydrogenase , pkc ) , pro - oncogene ( c - myc , c - fos , etc . ) , gap junction and protein synthesis , and so on 为了揭示工频磁场生物效应的机制，尤其是与肿瘤发生发展的关系，人们开展了较为广泛的体内及体外的实验研究，涉及到各种生物酶类( odc 、琥珀酸脱氢酶、 pkc等) 、原癌基因( c - myc 、 c - fos等) 、细胞间缝隙连接及蛋白合成等方面。
In order to study the regulatory mechanism in gametogenesis of loxoblemmus doenitzi , the special expression of c - kit and c - myc is investigated by immunohistochemical method . the results show that there are kit positive protein granules on the cellular membrane of spermatomeres from metaphase i to anaphase ii , and in the head of mature spermatozoa not only in the testis but also in the spermatheca 结果表明， kit在多伊棺头蟋精子发生中期至后期的精母细胞膜上有阳性颗粒，在精巢和受精囊内精子头部残留的细胞质内也有阳性表达；但在出生后卵子发生过程中，发育中的卵母细胞上无阳性表达。
34 we further investigate the apoptosis related gene . using different concentration of il - 16 treat jurkat cells in 1640 without serum , the expression level of bid and c - myc increase together with the increase il - 16 concentration . these suggest that bid and c - myc may take a part in the apoptosis induced by high concentration of il - 16 结果表明，在无血清培养jurkat细胞24hr后，随着rhil ? 16浓度的增加， jurkat细胞的凋亡程度也增加，与之相应， bid和c一myc的表达水平也增加，这表示bdc m厂可能参与il叫6诱导的扣水ct细胞凋亡的调节。
However , kit protein doesn " t exist in oogenesis after birth . there are also c - myc positive protein granules in the spermatomeres from metaphase i to telophase ii , in the spermatozoa and in the secretory cells of the spermatheca . c - myc protein expresses not in the normal follicles , but in the follicular cells of the apoptotic ones Myc在处于减数分裂的中期至末期的精母细胞的细胞质或细胞核内呈阳性表达，在减数分裂后形成的精细胞和受精囊囊壁的上皮细胞的细胞质内也有阳性表达：但在出生后的卵子正常发生过程中无阳性表达，然而在凋亡卵泡的滤泡细胞的细胞质内有阳性表达。
However , only c - myc protein was slightly increased in cells transfected with vector alone and other proteins expression level were the same as conditions in the absence of tgf - 1 . in addition , hepg2 cells transfected with pcdnas . 1 / ha - axudl plasmid were arrested in gl phase compared with the cells transfected with vector alone 另外，流式细胞仪检测表明， ha一axudi蛋白的过表达细胞组较空载体转染细胞组， g0 / g1 ( % )上升，而s ( % )下降，说明细胞阻滞于g1期。
On the other hand , the expression is repressed by the proto - oncogenes n - myc and c - myc , and in some cases the expression is also repressed by testosterone or dihydrotestosterone ( androgen ) at mrna level infering from all the references that can be obtained , the functions of ndrg family may be involved in cellular differentiation events > maintaining the balance of cell redox potential ( in oxidative stress condition or biological conversion process ) , or counteracting cell malignant transformation , and so on 综合己有的文献，推测ndrg家族可能参与细胞生长分化、维持细胞氧化还原电势平衡（氧化应激或生物转化） 、阻止肿瘤细胞恶化等。因此，研究其在细胞中的具体功能以及可能的信号通路将具有非常重要的意义。深人研究ndrgz在体内的组织细胞和亚细胞水平分布特点及其功能，需要高效价和高特异性的ndrgz抗体。
