Mistakes in the dna reconstruction process , Dna重组过程中的错误
Scbi 536 basic principles of animal behavior Dna重组工程原理
The committee recommended that recombination dna work at princeton be considered in this same light 委员会建议用同样的观点来考虑在普林斯顿的dna重组工作。
This work is the analysis of metabolic pathway and designing rational genetic modification to optimize cellular properties by using principle of molecular biology 本研究根据代谢工程原理系统分析了细胞代谢网络,并利用dna重组技术合理设计细胞代谢途径及其遗传修饰,进而完成细胞特性改造。
In 1987 , the notion of a splicing system was introduced by tom head in [ 3 ] as a mathematical model of restriction enzyme digestion and subsequence religation in the recombination of dna molecules 1987年, tomhead发表了一篇论文,引入拼接系统( splicingsystem )的概念作为限制酶与dna (脱氧核糖核酸)作用、进行dna重组的数学模型。
The expressive gene fragment was 333bp long . analyses of the tcr y fragment shpwed that it contained three epitopes . it was showed that specific anti - idiotypic antibody could be found since four weeks after the first immunization and came to the climate on the sixth week . the antibody titers of the same time were higher in group pcdna3 / tcr y - il - 2 than in group pcdna3 / tcr y ( po . 01 ) . the highest antibody titer was 1 : 640 in the group pcdna3 / tcr y - il - 2 , whereas the highest was 1 : 160 in the group pcdna3 / tcr y . there were mrna expression in skeletal muscle cell could be found in group pcdnas / tcr y and group pcdna3 / tcr y - il - 2 on fifth day after inoculation 用tcryvi独特型dna重组载体作为疫苗免疫小鼠,结果表明pcdna3汀cry组及pcdna3厅cry ? il ? 2组小鼠1血肩中全部产生了特异性抗独特ffo抗体,抗体滴度在第4周开始增高,第6周时达到高峰。在同一取血时间, w儿1 ry一几一二组小鼠抗体滴度均高于pcdna3 tcry组( p 0 1 ) 。 pcd a3 tcry组小鼠抗体滴度最高达1 : 16小pc 。
However , its wide application in manufacture has always been restricted by such a question as low phytase - producing level of wild strains , so before the wild strains which can produce acidic phytase are widely used , their phytase activities must be improved by various means , in which using an efficient expression system to express heterogenous phytase is a main consideration 植酸酶主要存在于微生物和植物中,动物体内也有少量存在,但真正具有开发价值的主要是微生物产生的植酸酶,尤其是曲霉产生的酸性植酸酶。国外早在1991年就成功地利用dna重组技术获得第一株酸性植酸酶的工程菌,并已投入商品化生产。
Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells , we design a pair of particular primers , and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic . the expression plasmid was identified with pcr and dna sequencing . pbv220 - r hpf4 was transformed into e . coli dh5a , bl21 ( de3 ) and induced by increasing the temperature to 42 . we identified the expression protein by sds - page and western - blotting 目的1人血小板因子4 ( hpf4 )原核高效表达克隆的构建2重组hpf4的表达及分离、纯化工艺研究3重组hpf4的特性研究方法根据原核细胞表达真核蛋白的基因表达调控特点,设计合成一对特异引物,在pt7 - 7 - rpf4表达质粒的基础上,应用聚合酶链式反应( pcr )对其cdna进行改造,通过dna重组技术构建成重组hpf4原核表达质粒pbv220 - rhpf4 ,用快速pcr检测法、 dna测序分析,鉴定重组hpf4表达质粒的正确性。
The pil - 6 cdna fragment was inserted into pgex - 1 ? t plasmid to construct the expression plasmid pgpil - 6 , the recombinant plasmid was digested by bamh i and pst i to identify whether the pil - 6 cdna fragment was inserted into the plasmid in correct orientation , the pgpil - 6 was transformed into e . coli dh5 ? competent cells 用dna重组技术将已克隆的猪il ? 6cdna片断插入pgex ? 1 t质粒,构建了猪il - 6基因的原核表达质粒pgpil - 6 ,转化大肠杆菌,以bamhi酶切筛选、 psti酶切鉴定转化子,获得了含740bp插入克隆基因正确的重组子。
By recombinant dna techniques , the vp2 gene of gpv hi strain was fused in frame with 6his gene of prokaryotic expression vector pproex - htb . the recombinant expression plasmid of goose parvovirus vp2 gene pproex - htb - vp2 was transformed into e . coli dh5a and induced with iptg . sds - page analysis showed an induced expression product band about 72ku , which correspond to the sizes of vp2 , reported in the literature 利用dna重组技术,将其结构蛋白vp2基因亚克隆至原核表达载体pproex - htb , iptg诱导后成功表达出与预期大小相符的约72ku的融合蛋白,光密度扫描对表达产物进行初步定量,表明表达产物约占菌体总蛋白的14 。