An internal segment of the whig gene of s . griseus was amplified from plzl through pcr . the 304bp dna fragment was inserted into the ecori / bamhi site of e . coli - streptomyces shuttle plasmid pkc1139 , generating pkc1139 : : a whig , named plz107 , for gene disruption Pcr法克隆whig基因内部304bp片段，连接到大肠杆菌-链霉菌穿梭质粒吓0139 ，构建了基因阳坏用重组质粒贼q139 ： ：凸mg ，命名plz107 。
14 out of 25 strains of biocontrol agent streptomyces showed chitinolytic activity by biological assay . these 14 strains of biocontrol agent streptomyces were detected for chitinase gene using pcr primers derived from the conservative sequence of 17 cloned chitinase genes from streptomyces 此方法相对于几丁质酶的传统生物学检测，具有更好的准确性和灵敏性；而且与生物学检测相比，使用分子检测省时省力。
Fr - 008 and streptomyces griseus imru3570 are two independently isolated producers for a similar heptaene macrolide antibiotic . comparative studies between the two producers at chemical and biological level are the main task of the present work , no obvious difference was observed on the hplc profiles 本文主要从化学和生物学两个角度分析和比较了这两个菌株的异同，并对抗生素的氨基海藻糖残基基因进行了克隆尝试。
A novel dna modification discovered in streptomyces lividans is different from dna methylation . this unusual modification causes wild type s . lividans dna sensitive to site - specific oxidative double - strand cleavage ( dnd phenotype , dna degradation ) 变铅青链霉菌的dna异常修饰系统是一种不同于甲基化修饰的新型修饰系统，它可使变铅青链霉菌dna在电泳时易遭到氧化双链切割( dnd表型， dnadegradation ) ( zhouetal . , 1988 ) 。
Using these vectors , expression of the pks gene was achieved both in streptomyces lividans and in e . coli in a heat - dependent manner , suggesting that the lambda promoter and temperature - sensitive lambda represser functioned in s . lividans as well as in e . coli 利用这些质粒在变铅青链霉菌和大肠杆菌中均表达出pks蛋白，两种宿主中的表达都是热依赖的。暗示噬菌体启动子和温敏型阻遏物在变铅青链霉菌和大肠杆菌中都具有功能。
Base on the successful expression of pks gene by cits857 controlled lambda promoter , an e . coli expression vector phz330 , an e . coli and streptomyces bi - functional vector phz1060 were constructed containing the dts857 - pr for inducible expression in both hosts . both vector 根据受cits857调控的p _ r能引导pks基因的成功表达，构建了大肠杆菌表达载体phz330以及在大肠杆菌和链霉菌两种宿主中都能表达外源基因的双功能表达载体phz1060 。
The entire functional dnd gene cluster was minimized to an 8 . 3kb insert in phz1904 , because phz1904 could complement the dnd phenotype of s . lividans mutant strain zx1 as well as streptomyces parvulus atcc12434 and streptomyces nanchangensis ns3226 , two naturally dnd - deficient streptomyces strains 将这个8 . 3kb的区域以整合形式导入无dnd表型的两个异源宿( ? )中，均使它们获得了dnd表型，表明这段8 . 3kb的区域包含了完整的异常修饰的功能。
