The genomic dna extracted from three rubber tree strains ( pr107 , rrim600 , gt1 ) was digested with hinckr . the southern blot was made with the same probe as above . the result showed that all the three strains turned up only one brand ( - - 4 . 8kb ) . the three strains had the same inherent background 用橡胶树pr107 、 rrim600 、 gti三个品系的基因组洲a分别经hinan酶切后，进行了southern杂交。结果表明，三个无性系都只出现一条杂交带，位置一’致（ 4
Pcr analysis showed that approximate 9 % tested plants produced the target band . a few of the 9 % plants produced the band in southern blot analysis . additionally , the number of inserted copies of dsg 10 was different in different transgenic plants 结果显示有9的再生苗为pcr阳性，对pcr检测阳性苗进行southem杂交分析，发现dsg10已整合入烟草的基因组中，而且在这些苗中dsg10片段整合的拷贝数不同，部分pcr阳性苗southern杂交结果却是阴性。
Pcr , pcr - southern blot analysis , southern dot blot analysis of lettuce dna confirmed that adw gene had been integrated into the plant genome . the results also showed that the transformation frequency of pb - adw was higher than that of pbg - adw , which suggested that camv35s promoter would be better than pi ii promoter in the case of transgenic lettuce ( 3 )细胞核载体pb - adw 、 pbg - adw均采用农杆菌介导法将adw导入莴苣，细胞核转化获得了生长良好的抗性莴苣植株，经pcr 、 pcr - southern 、 southern斑点杂交分析证实， adw基因已整合到莴苣基因组中。
The growing graphs of e . coli bl21 containing pbv221 and pbv221svhb according to the od600 values showed that the e . coli vgb + grew faster 4 the highly - efficient bivalent vectors which are named pgbif4abcvhb and pgbi4asvhbbt respectively , havfs been . su ? essful ] y constructed . the tobacco leave discs were transformed with agrobacterium tumefaciens carrying the plant expression vectors pgbif4abcvhb and pgbi4asvhbbt . 38 and 36 transgenic tobacco plants have been obtained respectively after kanamycin screening , pcr detection , southern blot analysiss showed that the foreign genes have been inserted into the tocacco plant genome . the expression of 16kd vhb protein in transgenic plants was proved by western blot 4构建了双价基因高效植物表达载体pgbif4abcvhb和pgbi4asvhbbt 。通过农杆菌介导法，将这两个双价基因高效表达载体导入烟草，分别获得了38 、 36株阳性转基因植株。经pcr 、 southernblot分子生物学方法分析，证明vgbm ， bt基因、 bt - cpti基因已整合到烟草基因组中， westernblot证明vgbm成功地表达出16kd的vhb蛋白。
There is only one copy in thellungiella salsuginea genome according to southern blotting . analysis of mrna levels by northern blotting indicated that the expression of tstrx - h in thellungiella salsuginea leaves was difference after being treated with 200 mmol . l - 1nacl for different hours and with different concentration of nacl for the same time Northen分析表明小盐芥在不同盐浓度和不同盐处理时间下的表达量明显高于对照： 200mmol . l ~ ( - 1 ) nacl处理盐芥随时间的延长tstrx基因在盐芥中的表达量会增加， 48hr达到最高。
Totally 99 transgenic rice plants from 125 resistant calli of 191 calli were obtained and pcr assay showed that 80 . 3 % of them were positive . the result of southern blotting analysis for primary plants revealed that each transgenic plant contained a average of 2 . 5 copy of t - dna 对抗性植株进行pcr扩增检测，结果表明有80 . 3的抗性植株为阳性植株； southern杂交结果进一步证明了质粒的t - dna已经整合到水稻的基因组中，拷贝数为1 4个，平均每棵转化植株有2 . 5个拷贝。
2 sequences were analysed and compared in genbank : ptfl is 494bp and ptf2 is 763bp in longth , the deduced proteins consist of 88 and 172 amino acids respectively , these cdna - clones encode the same gene - fe ( ii ) - transporter . 3 southern blotting showed that fe ( ii ) - transporter was a single copy in malus xiaojinensis genome and it was also existed in malus baccata and malus zumi 2 、根据测序的结果，将所得序列在genbank中进行同源性比较，结果如下： ptf1长为494bp ， ptf2长为763bp ，分别编码88个和172个氨基酸，属于小金海棠fe ( )转运蛋白基因的两个不同长度的基因片段，是同一个基因。
