Among 12 b - all patients there were two cd10 + , five cd19 + , two cd20 + and two hla - dr + . anti - cd3 and anti - cd4 dots of one patient showed significant difference using optics microscope observed . these cells were characterized by regular shape with dense chromatin on the anti - cd3 淋巴瘤病人胸水中有大量幼稚淋巴细胞，其中3例cd3 、 4例cd4 ，属t细胞淋巴瘤， 3例cd19 、 7例cd20 、 3例cd22 ，属b细胞淋巴瘤，阳性点上的细胞与非阳性点上细胞无论在细胞数量、细胞大小、形态规则性上都有明显的不同，用显? 2 ?微镜观察更是明显。
The recombinant was identified by dual enzyme digestion and the direction of cd40 / pcdna3 was analysed with t7 primer . after being packed by lipofectaminetm2000 , the recombinant was transfected into b lymphocytes . cd40 expression on membrane , cell proliferation and antibodies concentration were detected with flowcytometry , mtt colorimety assay and el1sa , respectively 以脂质体为介质瞬时转染健康人及sle患者b细胞系，利用流式细胞技术检测膜cd40的表达情况，并利用mtt比色法检测细胞的增殖能力， elisa法检测培养液的ig浓度，以研究b细胞在cd40被抑制以后增殖能力、抗体分泌的改变。
