And hematopoietic progenitor assay was used to examine hematopoietic differentiation . the effect of mbmec - cm when combined with cytokines or mouse fetal liver stromal cell - conditional medium ( mflsc - cm ) on inducing es cells differentiation into hematopoietic cells was detected . the hematopoietic reconstitution of es cell - derived hscs was detected in vivo in irradiated recipient female mice 实验根据造血集落的形成、造血干祖细胞特异抗原和造血相关基因表达等方面检测诱导生成的细胞是否含造血干祖细胞以及生成造血干祖细胞的数量，并且检测诱导生成细胞的造血重建功能。
The thesis is divided into four sections : in the first section , the author exames the long history and culture of the traditional settlement in shaoxing . having analyzed the effect of the economical structure changing to the form and development of the town , it is expressed the special structure of the waterland and the architectural enviroment in shaoxing 全文共分四部分：第一部分简述了绍兴传统水街民居集落的悠久历史和传统文化，分析经济结构的变更对集镇形成和发展的影响，并展示了绍兴地区独特的水乡集镇格局和建筑空间环境。
After 4 days of growth , the intact inner cell mass ( icm ) were separated with 0 . 05 % trypsin - edta and replated on feeder layer in dmem containing 15 % serum , 0 . 1mmol / l nonessential amino acids , 0 . 1mol / l 2 - mercaptoethanol , 2mmol / l glutamine , 100 units / ml of streptomycin and 100 units / ml of penicillin . after 4 - 6 days 5 es cell colonies were selected and expanded in which alkaline alkaline phosphatase was detected ( 2 )小鼠囊胚或内细胞的培养和es细胞的分离培养了156个不同品系的小鼠囊胚，经过3 ? 4天培养后，将增殖出来内细胞团用机械法结合胰酶- edta处理，离散后培养于mef饲养层上， 4 6天后有5例出现了es细胞样集落。
( 5 ) when pgcs were cultured on different feeder layers , the better results were got if pgcs were cultured on sto and mef1 feeder layers , when pgcs were cultured on thawed sto feeders , pef , inactivated pef feeders , 0 . 1 % gelatin , we can not get typical eg colonies ( 5 )用不同饲养层培养pgc ，实验结果证明pgc在新鲜制作的sto和mef ~ 1饲养层上，可以得到传3代以上的eg集落； pgc在铺有解冻的sto饲养层细胞、 pef或丝裂霉素c处理过的pef 、涂有0 . 1明胶的4孔板上，一直没有得到典型的eg集落。
