转化效率

• Acetosyringone ( as ) could dramatically improve transformation frequency , its suitable concentration is 20 mg . l " 1 . before inoculated with agrobacterium , calli should be precultured 1 ? days , and the appropriate inoculation time is 4 ~ 8 minutes , and then cocultured for 3 ~ 4days . then the cocultured calli were transferred to ms medium containing 400 mg . l " 1 cef
  7为宜；侵染前预培养1 2天，可显著提高转化效率；侵染时间以4 8分钟为宜；共培养时间3 4天较适合；转化后暗培养对转化效率没有影响；选择方法以延迟选择15天选择转化率最高。