The result of sequencing analysis showed that the nucleotide and deduced amino acids sequences had high identity with the corresponding parts of - glc cdnas of other published plants . the results indicate that the special pcr product is a cdna fragment of - glc of tea plant 将扩增片段克隆到pmd18 - t上进行序列测定和分析，结果表明，此片段核苷酸序列与其他植物-葡萄糖苷酶基因cdna相应序列有很好的同源性，推断该pcr特异产物为茶树-葡萄糖苷酶基因cdna片段。
A . niger m - l which was screened in our laboratory produced a strongly a - transglucosidase . in this paper , studies on the fermentation conditions , purification and characterization of a - transglucosidase and its necessary groups was carried out in this dissertation . the main reports were as following : the fermentation conditions in shaking flasks were investigated by the method of single - factor analysis , the suitable main medium was achieved : which contained 4 % a , 2 % b and 1 % g ; the a . niger m - l was inoculated into 100ml medium in flask , shaking in 33 c at 140r / min for four days , with initial ph6 . 5 and 6 % inocula volume ; adding 0 . 1 mmol / l methyl a - d - glucopyranoside had inductive effect on enzyme formation , the a - transglucosidase activity amounted to 296 . 05u / ml 本研究以黑曲霉m - 1为出发菌株，对其-葡萄糖转苷酶的产酶影响因素、纯化、酶学性质以及必需基团进行系统的研究，结果如下：通过对影响黑曲霉m - 1产-葡萄糖转苷酶的单因素分析，得液态发酵生产-葡萄糖转苷酶的最适产酶条件为： 4 a ， 2 b和1 g ；在33 ，起始ph值为6 . 5 ，转速为140r min ，接种量为6 ，装液量100ml条件下，发酵4 . 0d ，酶活力达296 . 05u ml ，添加0 . 1mmol l的酶作用底物甲基- - d -葡萄糖苷对产酶的诱导作用最大。
