It was observed that the inclusion complexes of 3 - cyclodextrin and its polylactide - garfted copolymer with insulin could be formed and the inclusion behaviors were affected by the amount of 3 - cyclodextrin , temperature as well as ph value . the formation mechanism of the supramolecular systems between 3 - cyclodextrin and insulin was the similar to that between 3 - cyclodextrin and the above - mentioned aromatic amino acids . because of the three - dimensional structure of insulin and the exterior polar groups of its aggregates , the molecular recognition and inclusion of 3 - cyclodextrin with insulin were hindered 由于受主客体之间的尺寸匹配性的影响， ?环糊精与这三种芳香族氨基酸形成了不同包合比的包合物， ?环糊精分别与l ?苯丙氨酸和l ?酪氨酸形成了1 ： 1的包合物，而与l ?色氨酸却形成了2 ： 1的包合物，各包合物的稳定常数也有所不同。
The inclusion behaviors of b - cyclodextrin with some aromatic amino acids such as l - phenylalanine , l - tyrosine and l - tryptophan were studied via ultraviolet and fluorescent spectrometry . the results indicated that inclusion complexes of - cyclodextrin with aromatic amino acids could be formed and the inclusion behaviors were affected by the amount of - cyclodextrin , temperature and ph value 本文研究了?环糊精与芳香族氨基酸l ?苯丙氨酸、 l ?酪氨酸和l ?色氨酸之间的包合作用，考察了?环糊精用量、温度、时间和ph值等因素对包合作用的影响。
Addition of iptg to growing culture of the lysogen induces t7 rna polymerase , which in turn transcribe the target dna in the plasmid . in the presence of glucose and appropriate conditions such as temperature and concerntration of iptg , a 52kd protein with tryptopanase activity was expressed 摸索发酵条件，如改变培养温度和iptg浓度等，发现在30培养条件下， 0 . 2mmiptg诱导时，发酵液中的吲哚含量最高，表明低浓度的诱导剂或低温诱导有利于表达出有活性的色氨酸酶。
