This modification includes : ( 1 ) selecting two important molecules as candidates , ( 2 ) choosing a promiscuous t - cell epitope , and two b - cell epitopes or conserved amino acid sequences from the two important molecules , ( 3 ) connecting them adequately through analysis by the molecule designing software . therefore , the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing . based on the molecule designing methods , the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse , as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected 本研究以蛋白质分子设计的理论和方法研究避孕疫苗，将sp17和cyritestin关键表位和牛核糖核酸酶非选择性th细胞表位合理组合，获得新抗原- 35肽序列；并在合成、纯化后分别与弗氏佐剂、免疫刺激复合物( iscoms )混合后免疫不同遗传背景的雌性小鼠，观察血清和生殖道内的特异性抗体滴度的动态变化、生育力的改变以及免疫后小鼠重要脏器的组织病理学改变：以及在ivf下，新抗原的特异性抗血清对精卵相互作用的影响及抗原在精子表面的特异性定位。
The subchronic toxicity experiment studied the effects of different pyrethroid concentration ( 1 / 10 1 / 20 , 1 / 40 1 / 80 of 96hlc50 ) on the behaviour , growth , haematological biochemical indices and pathology of carp in 60 days . the results showed that pyrethroid had no effect on the body length while had obvious influence on the body weight intake of food and swiming , some carp then showed intermittence toxic symptom until death 亚慢性试验研究了鲤鱼在不同低浓度拟除虫菊酯( 96hlc _ ( 50 )的1 80 、 1 40 、 1 20 、 1 10 )下60天暴露实验中，其行为、生长、血液生理生化指标和组织病理学所发生的变化和受到的不同影响。
