Two degenerate primers were designed and synthesized according to the highly conservative sequences among the known - glc genes . a cdna fragment of 208bp was amplified by rt - pcr , which was subsequently cloned into pmd18 - t vector for sequencing analysis 利用genbank中已经登录的其它植物中该酶基因的保守序列，设计一对简并引物，采用rt - pcr技术，从茶树扩增出208bp的cdna片段。
These results revealed that halocin c8 is cytocidal and its primary target might be located in the cell wall of the sensitive cells . two degenerate oligoxynucleotide primers ( c8 - 2 , c8 - 4 ) were designed according to the n - terminal amino acid sequence of halocin c8 根据嗜盐菌素cs的n端氨基酸序列设计了一对简并引物( cs一2 ， cs礴) ，利用这对引物从as7002的总dna中扩增出halcs的部分序列。
