The main research contents and results are as follows : ( 1 ) disc papers and oxford cups were used to ascertain bacteriostatic effect of hongqu . using 70 % ethanol and water as solvent , the extraction inhibited bacillus subtilis , bacillus cereus and staphylococcus aureus and had no effect on e . coli , salmonella . typhi , yeasts and algae 主要研究内容和结果如下： ( 1 )利用滤纸片法和管碟法确定了红曲的抑菌谱：红曲70乙醇和水溶液浸提物对蜡样芽孢杆菌、枯草芽孢杆菌等革兰氏阳性菌具有较强的抑制作用，对革兰氏阴性菌、霉菌和酵母菌没有抑制效果。
3 . the cloning of a - amylase gene : the methods of shotgun , pcr and rt - pcr were selected to clone a - amylase genes from bacillus subtilis hn503 , xanthomonas campestris pv . campestris 8004 and aspergillus oryzae , hn504 the recombinant plasmid with cloned gene was designated as phn504 , phn503 and phn8004 本研究分别选用了鸟枪克隆法、 pcr和rt - pcr克隆法，成功地克隆到枯草芽胞杆菌( bacillussubtilis ) hn503 、野油菜黄单胞菌( xanthomonascampestrispvcampestris ) 8004和米曲霉( aspergillus口尽留‘ ) hn504的价淀粉酶基因。
