2 . pcr test that - actin is included in the library and the titer of amplified cdna library is 1 . 0 1010 . the result of the third part : 1 2 .以p一actin为引物, cdna文库为模板进行pcr反应显示: p一actin的318bp扩增条带清晰。
Then cdnas and puc18 vectors were linked by t4 dna ligase and transformed into e . coli strain dh5 - alpha to generate cdna library that size is 4 . 9 l06 recombinants 将cdna与载体连接,并导入dh5感受态细胞中,构建成cdna文库。
It demostrated that the library is of high quality and can be used as a valuable resource for studying gene expression and regulatory mechanisms 研究表明,所构建的文库质量较高,可以广泛用于草鱼免疫相关基因的表达和调控研究。
At the end of year 2005 , the collection series was up to 73 kind of books , the " knowledge of ling nan culture " series had 38 kinds of books already 到今年底,作为学术性丛书的《岭南文库》已出书73种,而《岭南文化知识书系》也出版了38种。
A genome library of serratia marcescens cw - w - 90 - 3 strain was established by " shot gun " method and screened for phospholipase activity on lb - egg yolk plate 通过鸟枪法构建了粘质沙雷氏菌serratiamarcexcenscw - w - 90 - 3菌株的基因组dna文库。
3 fifty candidates proteins possible interacting with the nthk1 protein were screened from the tobacco cdna library through the yeast two - hybrid system 3利用酵母双杂交技术从烟草的cdna表达文库中筛到50个可能与nthk1的相互作用的蛋白基因。
266 old chinese medical books were lost in china but still stored at 18 libraries in the world , of which 206 are collected at the japanese cabinet library 世界上有18家图书馆收藏着中国散佚的266部中医古籍,其中的206部是日本内阁文库的藏书。
He has presided over the translation of " foreign law works " and drafted such works as general rules of civil law , company law and trust law 他主持了"外国法律文库"的翻译工作,承担了《民法通则》 、 《公司法》 、 《信托法》等多项法律的起草任务。
It ' s imperative to establish the rh gene library based on han nationality corresponding primer designing and gene typing method suitable for chinese 建立以汉民族为代表的rh基因文库,以及相上的引物设计和更适合中国人群的基因检测方法,势在必行。