The antigenic and genetic variability of porcine reproductive and respirators syndrome virus ( prrsv ) isolates in china were studied by immunofluoresent monolayer assays ( 1fma ) and restriction fragment length polymorphism ( rflp ) of reverse transcription ( rt ) and polymerases chain reaction ( pcr ) amplified - prrsvorfs fragments among 8 chinese isolates 本研究通过对猪繁殖与呼吸综合征病毒( prrsv )国内分离毒株的gp3 、 gp5和n蛋白的抗原性比较及其orf5和orf7遗传变异性分析，系统研究了国内分离毒株的抗原特性和遗传学差异。
The expressed product was lysised with supersonic wave and purified by sds - page . elisa analysis revealed that the antigenicity of the vpi protein has been detected . the forth part - detection of aev - nh937 strain by in situ hybridization ( 1sh ) - probe was labelled with digoxigenin ( dig ) . then the probe hybrid with 5d , 10d , and 20d postinfection brain tissue of chicken . the results of the ish showed that the positive signal was found in 3 cases , while control group was negative . there has been a reasonable correlatien between this method and other detection test 经超声波裂解，用尿素溶解包涵体，电泳纯化后，利用elisa检测vp _ 1外壳蛋白，表明具有一定抗原性；第四部分：应用原位杂交检测aevi用dig标记的探针与sd 、 10d 、 20d的攻毒鸡脑组织杂交，来检测那v的rna 。
The result shows that a vvibdv strain was obtained , the above work lay a important role for further studying on the molecular biological mechanism of antigenic drift and virulence variation of ibdv , molecular epedimiology , it also provided the basis for recombinant and gene deleted vaccine of ibdv 本实验可以帮助我们进一步探讨ibdv抗原性漂移和毒力变化的分子生物学机制，追溯ibdv的起源，理解病毒的传播方式。同时也为研制开发基因重组疫苗和缺失疫苗打下一定的基础。
In order to express the recombinant peptide of both gp52 and pp150 oterminal peptides from human cytomegalovirus ( hcmv ) , which seem to show good antigenicity . recombinant dna technology was used to construct recombinant plasmid , which was transformed into the pichia pastoris to express the interesting peptide 为了表达人巨细胞病毒( humancytomegalovirus ， hcmv )中抗原性较强的两段蛋白片段? gp52c末端和pp150c末端的嵌合肽，用基因工程技术构建适于酵母表达系统的重组表达质粒。
Main methods and results are as followed : 1 epitope analysis of agonist - binding region of nrla physicochemical properties and antigenicity of two agonist - binding regions of nrla were analyzed through bioinformatics : domain p1 containing 151 amino acid residues preceding the first transmembrane domain of the human nrla , domain p2 with 144 residues following the third transmembrane domain . four parameters including hopp - woods and kyte hydrophilicityjanin accessibility , karplus - schulz flexibility , and welling antigenicity were used to determine the antigenic sites , and prosite programme and chou - fasman method were employed to analyze their related sequence motif and the secondary structures 用goldkey软件分别选取公认的hopp等与kyte等亲水性参数、 jain表面可及性参数、 karplus - schulz主链柔韧性参数及welling抗原性参数对p1 、 p2两个多肽片段进行参数分析。并采用通用的prosite程序与chou - fasman方法比较分析p1 、 p2多肽片段的氨基酸位点与二级结构特征。综合判定两个多肽片段的抗原性及其位点，结果认为p2抗原性强于p1 。
The results revealed that antigenic drift had occurred in virus strains of the same h9n2 subtype in the north of henan province during 1998 - 2002 . h9n2 subtype aivs were stable from 1998 to 1999 , since the mutual protection between 98a5 and 99s approached to 100 % , which was consistent with results in hi and vn tests 1998年和1999年h _ 9n _ 2亚型禽流感病毒较稳定， 98a5和99s毒株间的保护力接近100 ，在hi 、鸡胚中和、细胞中和试验结果的相关性均在0 . 74以上，表明两毒株间的抗原性相近。
