Objective the origin and the nature of the hodgkin and reed - sternberg ( h / rs ) cell of hodgkin ' s lymphoma ( hl ) has been attracting a lot of medical researchers engaged in studying it , for its character by scattered large atypical cells residing in a complex admixture of inflammatory cells . great improvement has been made since a new method of isolation of single h / rs cells from a frozen section had been set up by kuppers in 1994 , and many studies have approved of the b - cell derivation of h / rs cell . lt has been reported that h / r - s cells might partly be originated from b - cell in our research before , but at the same time , we also found that only 18 . 8 % of h / rs cells express cd20 , 31 . 3 % of immunoglobulin heavy chain ( igh ) rearrangement have been revealed 目的霍奇金淋巴瘤( hodgkin ' slymphoma ， hl )由于它的恶性肿瘤细胞? h rs细胞一般只占肿瘤组织的极少部分(不到1 ) ，且散在分布在背景细胞间，因此对于h rs细胞的来源和性质研究一直是人们探索的目标。 1994年德国科隆大学k ppers等发展了一种从冰冻组织切片上用显微操作仪挑取单个h rs细胞的显微切割方法进行h rs细胞基因分析后，人们对h rs细胞来源的研究有了突破性的进展，多数支持b细胞来源。我们的前期研究也支持部分h rs细胞为b细胞来源，但我们发现只有18 . 8的h rs细胞表达cd20 ， 31 . 3的hl检测到igh基因重排克隆性条带。
