Mdj - 01 strain was compared with senzhang strain in biological characters and complete genome sequence . all helped to ascertain the cause for tick - borne encephalitis erupted in china northern eastern in late years , to study on the complete cdna of tbev , genome structure and function , diagnose , prevalence and therapy . the 12 subtype - special amino acids but 486 amino acids were n ' t reported , and were important to identify tbev strain isolated newly with which subtype 本试验对mdj - 01株和森张株进行了生物学性状和基因组全序列的比较，这将有助于阐明近年来tbe在我国东北再次发生流行的原因，同时为tbev全长cdna克隆、基因组结构与功能、诊断、防治等研究打下基础。
The sucking mouse brain were inoculated with mdj - 01 strain to make electron microscopic examination , results showed that the virus was a spheral particle with membran which had a diameter of about 40 nm . by indirect fluorescent antibody test mdj - 01 strain was identified with tbev . a part of region encoding e protein was expanded by rt - pcr and sequenced . the nucleotide sequences of two strain viruses were compared with sequences in genbankjsequence homology analyses revealed mdj - 01 strain and senzhang strain had the highest homology with tbev oshima5 - 10 , respectively , which were 95 % , 94 % . mdj - 01 strain was identified with tbev again 应用间接免疫荧光试验进行血清学鉴定，结果表明mdj - 01株为tbev 。通过rt - pcr技术扩增部分e蛋白序列并测序，在genbank上进行同源性比较，发现mdj - 01株和森张株与tbevoshima5 - 10株的同源性最高，分别为94 、 95 ，从分子生物学水平上进一步证明mdj - 01株病毒为tbev 。在鉴定的基础上，本实验对两株病毒进行了核苷酸全序列测定。
By transformation with the genes . plant disease biocontrol bacteria bacillus subtil is aplls and b . megaterium ap25 were isolated from wheat field soils collected from south australia and tai an . enzyme activity analysis on chitin agar and abp media showed that b . subtilis aplls secreted chitinase and b . megaterium ap25 secreted endoglucanase , respectively 测序后在genebank上进行序列比较，该基因片段同编号为2634966的枯草芽孢杆菌全序列的2599451到2812870 (功能未知)有85的同源性，但同已发表的13种几丁质酶的基因(包括枯草芽孢杆菌几丁质酶基因)的同源性很低，只有30 。
Rt - pcr was used to amplify the cdna of the genome . these cdna fragments were cloned into the plasmid pucm - t . the result indicated that the seguence of the genome was obtained . the genome of aev - nh937 composed of 7055 nucleotides , potentially encodes a polyprotein of 2134 amino acids . the genome of aev - nh937 has 94 . 3 % nucleotides identity with the calnek vaccine strain of aev 把它们分段克隆在pucm - t载体上，经序列分析，获得了aev - nh937毒株的基因组全序列及推导的氨基酸序列，基因组全长为7055个核苷酸，与calnek疫苗株具有94 . 3的同源性，编码一个含2134个氨基酸的多聚蛋白。
So , combined examination of mutation of hbv c gene , the concentration of cytokines and t lymphocyte subpopulations is very significant to real ise the relationship between hbv c gene mutation and the cellular immunity of the host , and also can offe r the basis to further announce the pathogenesis of chronic hepatitis b and individual diagnosis and treament 因此联合检测hbvdnac基回全序列、血清细胞因子水平及t细胞亚群，可以全面了解基因的变异情况，综合分析变异与细胞免疫功能的相关性，并为进一步揭示慢性乙肝的发病机理及个体化诊断和治疗提供依据。
A small cryptical plasmid pefr was isolated from enterococcus faecium strain df101 . the complete sequence analysis of the plasmid show that it consists of 3176 bps , which contains four putative orfs . orf1 encodes a putative protein and is highly similar to repa which functions in replication 从屎肠球菌df101菌株中分离到隐秘的小质粒pefr ，全序列分析显示质粒pefr由3176bp组成，编码四个推定的orf ， orf1编码的一个推定的蛋白和复制有关的repa有很高的相似性。
