Immunohistochemistry and western blotting further confirmed that the prepared antiserum could recognize not only the prokaryotic expressed 6his - agtc , but also the endogenous agt protein in many tissues of rat and human Western印迹分析及免疫组化显示，所制备的抗血清不仅可识别原核表达的agt片段，而且能够识别大鼠及人多种组织中内源性agt蛋白。
P - liquid scintillation counting was used for analysis of erk and immunocytochemistry was used for the analysis of c - jun . all the data were analyzed with statistical treatment . the results were showed as follows : 1 分别做p液闪计数观察erk的活性表达，做westernblot观察jnk的表达量变化，免疫组化观察cjun阳性信号的表达，并进行统计学处理，结果如下： 1
Expression of nos and et were observed in experimental groups . microscope showed a characteristic change of ischemic cell damage for example : loss of glycogen . break clumping and margi - nation of nuclear chromations , mitochondria with the ap - pearnce of amorphous dense bodies ( 3 )免疫组化染色发现： et在1h组和2h组的缺血区和交界区为阳性表达，正常区和对照组为阴性表达。
Human granulosa cells isolated from follicular fluid of 16 patients received ivf or icsi were cultured in tcm199 medium . granulosa cells were examined to detect fshr by immunobiochemistry assay and rt - pcr . 3 对16例接受体外受精患者卵泡液中分离的卵巢颗粒细胞进行体外培养，利用免疫组化法和rt pcr法检测颗粒细胞的fshr的蛋白表达和mrna的表达，鉴定体外培养颗粒细胞的纯度； 3
Immunohistochemistry staining showed that induced different - passage mscs expressed neurofilament ( nf ) and neuron - specific enolase ( nse ) . special nissl bodies were obvious in the neuron - like cells by histochemistry staining 免疫组化法检测发现这些神经细胞强表达神经丝蛋白( neurofilament ， nf )和神经元特异性烯醇化酶( neuron - specificenolase ， nse ) ：组织化学法检测可见神经元特有结构尼氏体。
To investigate the relationship between aes / tle1 and tumors , the expression patterns of aes / tle1 were detected in samples of gastric cancer , cervix cancer , hepatoma and corresponding non - tumor tissues by immunohistochemistry assay 为探讨这两种分子与肿瘤的关系，我们通过免疫组化方法观察了宫颈癌、胃癌及相应正常组织中这两种分子的表达情况，发现胃癌组织中aes高表达，宫颈癌组织中tlei高表达。
Much difference of the t cell subpopulations was observed among individuals after immunization . the percentages of cd4 + , cds + or cd4 + / cd8 + t cells of the animals immunized with dna vaccines increased at different extents compared to the pires1neo - immunized or mock - immunized animals 与空白对照组和空质粒p旧esineo免疫组比较，各重组质粒免疫组动物的cd4 、 c08及cd4 ic08 t细胞有不同程度的升高。
