4 . to measure the expression of follicle stimulating hormone receptor ( fsh - r ) on cultured granrlosa cells and to study the correlation between the expression and ivf - et outcome ; to investigate the physiological mechanism of reactivity of the ovary to productive hormones in order to give a rich theoretic base on coh 4 、测定培养的颗粒细胞上fsh - r的含量，并研究其与ivf - et结果的相关性，从而进一步探讨卵巢对促性腺激素反应性的生理机制，为控制超排卵方案提供更广泛的理论基础。
Anti - p21 mouse monoclonal antibody from beijing zhongshan biotechnology anti - mouse or anti - rabbit igg secondary antibody from santa cruz biotechnology ly294002 from sigma biotechnology tritonx - 100 from boehringer mannhein gmbh fluorescein isothiocyanate ( fitc ) conjugated anti - mouse igg antibody was purchased from beijing zhongshan biotechnology hepes from e . metck darmstadt methods superovulation and collection of eggs for superovulation , female kunming mice 4 - 5 week old were injected with pregnant mare serum gonadotropin ( pmsg ) , and after 46 - 48 hours with human chorionic ginadotropin . ( hcg ) . one - cell fertilized eggs were collected on the next day from oviduct of females 取4一5周龄成熟雌性昆明系小白鼠，腹腔注射pmsg (孕马血清促性腺激素) 10iu ， 46一48小时后腹腔注射hcg (人绒毛膜促性腺激素) 1oiu ，将注射hcg后的雌鼠与8周以上的成熟雄鼠合笼交配，次日检察阴栓，将查到阴栓的雌鼠处死，取输卵管于mz培养液中，解剖镜下撕开壶腹，释放细胞团，然后用300林岁nil透明质酸酶消化去除颗粒细胞，口控吸管将卵细胞在m :中反复清洗，然后置于孵箱中，根据时间点收集g2期细胞。
