This pcr product was cloned into a t - vector and resulted in a plasmid ply11 , which was used as the template to generate a rna probe of halocin c8 for screening the halc8 gene in the as7092 genome by southern blot analysis 将这段pcr产物克隆到t -载体上，形成质粒plyn ，测序结果表明pcr产物所编码的氨基酸是与halcs成熟蛋白的n端氨基酸序列是一致的。
This gene , artificially put under the control of camv 35s , was introduced into tobacco with the aid of agrobacterium , and the transgenic plants obtained were identified by gus staining , pcr and southern blot analysis 将arge与组成型启动子camv35s相连，构建植物表达载体，通过农杆菌介导转化烟草。经过gus染色、 pcr及southern鉴定获得了转基因植株。
7 herbicide resistant plants were obtained . pcr , pcr - southern blotting analysis showed that the target genes were integrated into the plant genome . the segregation ratio in t1 of two examed transformants was 3 : 1 通过对标记性状? ?除草剂抗性的活体及离体检验(氯酚红法) 、目的基因pcr扩增、 pcr - southern杂交等方法证实目的基因已整合到小白菜基因组中。
We produced transgenic tomato plants by an agrobacterium tumefacien - mediated method . pcr - southern blot and rt - pcr revealed integration of afgf gene in the plant genome and successful its transcription in transgenic plant cells 我们利用农杆菌介导的转化方法获得了afgf转基因番茄植物，并且通过pcr - southern 、 rt - pcr证明了afqf在宿主基因组中的整合及表达。
89 ) via agrobacterium mediation . pcr and southern blotting analysis showed that the cmo and badh genes were integrated into tobaccos " genome . betaine accumulation in transformed tobaccos was higher than that in control plants 89 ) ，获得卡那霉素抗性和潮霉素抗性植株， pcr和southern杂交均证明外源cmo和badh基因大连理工大学博士学位已整合到烟草基因组中。
The deduced amino acid similarity of pii among g2 , tl , e . coli , and b . subtilis et al . were 69 . 47 % . southern blotting showed that there may be 2 copies of glnb genes in the dna genome of g2 本文还在氨基酸水平上比较了菌株t1和g2的glnb与e . coli 、 kpneumonia 、 b . subtilis及一些联合固氮菌的glnb基因的同源性，结果表明它们在氨基酸水平上的相似性为69 . 47 。
2 . southern blotting analysis indicated that sod2 gene had been integrated into all of the transgenic plants ' genomes . the copy number of sod2 in transgenic plants was mostly 1 to 5 by southern analysis Southern结果表明，野生型植株(对照)没有明显的杂交信号，转基因株系有强阳性信号，转基因植株中插入外源基因的拷贝数不同，既有单拷贝的，又有两个拷贝及多拷贝的，拷贝数从1 - 5不等。
Whether the gene was transformed into the arabidopsis was confirmed by pcr - southern blotting , southern blotting and northern blotting . 1 . pcr - southern blotting showed that all kanamycin - tolerant plants had strong positive signals , and no signal was shown in wild type plants Pcr结果扩增出1 . 4kb的特异性条带，表明sod2已整合进拟南芥中， pcr - southern进一步证实了pcr结果的正确性。
When the seeds from to transgenic plants were cultured on medium supplemented with 50mg / l hygromycin , the ratio of germinated to non - germinated seeds is 3 : 1 , which is consisted with mendel ' s first law . southern blotting demonstrated that the svde gene had been integrated into the genome of tobacco plants 转基因植株的l代种了在潮霉素培养基上的萌发数与未萌发数的比值为3 ： l ，符合单基因的盂德尔分离规律。
Transgenic plants were comfirmed by molecular determination including pcr , southern blot and rt - pcr . 4 . functional analysis and iron content of transgenic plant crude proteins of transgenic plant were extracted from leaves , and then used to test its antibiotic activities 4 .转基因植株抗菌能力加强，铁含量得到提高提取转基因植株叶片粗蛋白，以大肠杆菌dhsq和酵母菌ahiog为试验靶标，侧定了转基因植株粗蛋白的抑菌活性。
