Spf chickens with 21 - day - old were infected subcutaneously with oil - emulsion vaccine of ibdv of germinal or cellcular and injected intramuscularly with different dosages bursin which gain through ultrafilter . lt is proved that bursin of chickens and ducks can both shorten the time of antibody induced against ibdv , raise the level of serum antibody . they make chickens obtaining strong immunocompetente in a short time . agp liters of the group of infecting 0 . 4mlcbs + ibdv of germinal and 0 . 8mlcbs + ibdv of cellcular or 0 . 8mldbs + ibdv of germinal and cellcular are higher than immune control group about 2 liters averagely . the chickens were inoculated with ibdv live vaccine mixed with the different dosages of lyophilized bursin by the eye drop method . the results sugest that cbs or dbs of different dosages can both improve the antibody inducation to different age chickens against ibdv . they may also alleviate the immunological injury of activated virus to bursa of fabricius . and promote the repairation of the lesion . it can be found that bs can raise body weight gain and feed coversion ratio 将超滤获得的法氏囊活性肽分别以不同剂量肌肉注射21日龄spf鸡，同时颈部皮下注射ibd胚毒或细胞毒灭活苗，结果表明：鸡、鸭法氏囊活性肽都能够缩短ibd油苗诱导产生抗体的时间，提高抗体水平，使鸡可以在比较短的时间内获得坚强的免疫。 0 . 4mlcbs胚毒组和0 . 8mlcbs细胞毒组或0 . 8mldbs胚毒和细胞毒组的agp抗体滴度平均比免疫对照组高2个滴度。将法氏囊活性肽与ibd活苗联合免疫鸡，结果表明：不同剂量的cbs和dbs都可以对不同日龄鸡ibd抗体的产生有不同程度的促进作用；还可以减少弱毒对鸡法氏囊组织的损伤，加快其修复。
In this study we had used the sensor to detect antibody - antigen reactions of fasciolsis , nd , ibd and eds . it is proved the sensor is fitted to use in detecting antibody - antigen reactions of these diseases . the results of detecting antibody - antigen reactions of fasciola gegatica , nd , ibd and eds by using the sensor basically conform to that with standard samples 在实验研究中，我们先后应用电化学免疫传感器测试了大片形吸虫，鸡新城疫( nd ) 、鸡传染性法氏囊病( ibd ) 、鸡减蛋综合征( eds )等的抗原抗体反应，结果与我们先前初步设定的判断标准基本相符。
Infectious bursal disease virus has been a great concern for the poultry industry for a long time , particularly for the past decade when its " re - emergence " in variant or highly virulent forms . in this study , two sets of primers ( pta and pts , ibda and ibds ) , flanking the hyper - variable region of vp2 gene , were designed to run a reverse transcription polymerase chain reaction ( primary rt - pcr ) and nested - pcr . both of these assays can amplify all of 12 reference strains which including pathotypes cibdv , vvibdv and vibdv , but not the 5 negative reference pathogens of chicken 结果12个参考毒株均能扩增出约679bp的目的片段，而阴性对照的常见5种鸡病病原：鸡新城疫病毒( ndv ) 、鸡传染性支气管炎病毒( ibv ) 、鸡传染性贫血病毒( caiv ) 、大肠杆菌和多杀性巴氏杆菌均没有扩增到任何片段；应用建立的技术对疑似ibd的34份临床病料进行检测，并同时在基础rt - pcr扩增的片段内设计另一对引物进行nested - pcr 。
