Total rna was extracted from pituitary of new butchered female goat for fsh - a and b subunit genes . cdna were synthesized by rt - pcr reactions respectively and these cdna were used as templates in pcr amplifications . the pcr products were 360bp and 390bp which just were the same length of the predicted goat fsh - a and p subunit genes 本试验是从新屠宰的母山羊脑垂体中提取总rna ，反转录获得cdna ，以此cdna为模板， pcr扩增目的基因片段，分别获得长为360bp的山羊fsh -亚基dna片段和长为390bp的山羊fsh -亚基dna片段，与预期的目的基因片段大小一致。
T level of male is much higher , but their p , lh , fsh levels are much lower than those of female . the change rate with aging in male is also relatively slower . the concentrations of ptc and acth are related with the memory functions and the levels of e2 , p , t , lh . , fsh are related with attention , memory , thought and executive functions 男性在wais智力测验项目、 toh测验中成绩优于女性；随龄增长，除知识以外的大多数认知功能均下降，但男性相对保持较好；男性神经内分泌指标的差异主要集中在性激素方面，男性t高于女性， p 、 lh 、 fsh却低于女性；随龄增长，男女的神经内分泌指标的变化基本一致，但男性的变化幅度相对较小；男女神经内分泌水平和认知功能的相关项目稍有不同，但两者的acth 、 ptc与记忆功能相关， e2 、 t 、 lh 、 fsh等性激素与很多方面的认知功能如注意、记忆、思维分析能力、执行功能相关。
