花粉管

• These results indicated that it is practicable inducing exogenous dna into wheat through pollen tube pathway to obtain taget plant . there are great potential power to improve wheat quality by this way
  本实验结果表明：利用花粉管通道法将外源dna导入小麦中获得目的性状的植株，从而改良小麦品质，在实践上是可行的，在生产中具有极大的潜力。
• Ecms associated with pollen tube growth include arabinogalactan proteins , extensin - like glycoproteins , proline - rich proteins , calmodulin , s - glycoproteins , pectins and those that specifically located in the ovary
  与花粉萌发和花粉管生长相关的雌蕊胞外基质种类主要包括阿拉伯半乳糖蛋白、类伸展素糖蛋白、富含脯氨酸糖蛋白、钙调素、 s -糖蛋白、果胶以及子房的特异性物质等。
• After treatment with bfa , immunoflurescence labeling and confocal laser scanning microscopy indicated that the cytoplasmic dynein intermediate chain - like protein in lily pollen tube was insensitive to bfa treating , but spectrin - like protein was sensitive to it under the same condition
  用bfa处理百合花粉管后，通过免疫荧光标记及激光共聚焦扫描显微镜观察发现类细胞质力蛋白中间链对bfa不敏感；同样的处理发现类红膜肽对bfa敏感。
• The results were as followed : pollen germination and tube growth were decreased in ga mutant , and increased in ga overexpression strains as compared to wild type , while pollen germination and tube growth were not affected in gp mutant
  结果表明：体外实验中gpa1突变体花粉的萌发率及花粉管生长速率均低于野生型花粉，而超表达g花粉的萌发率及花粉管生长速率则高于野生型及空载体对照； g突变体agb1 - 1与野生型花粉的萌发率及花粉管生长速率差异不大。
• Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l . cv hetao flesh , which was cloned into plasmid vector pmd - 18 - t . the clon of antisense orientation were selected , and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw , antisence expression vector pbinya was constructed . at the base that pollination and fertilization of cucumis melo l . cv hetao was studied , using pollen tube pathway transformate cucumis melo l . cv hetao , 76 fruit had been obtained , moreover , hardness and content of sugar were analysed
  本实验以河套蜜瓜果肉基因组dna为模板，用甜瓜acc氧化酶基因特异寡核苷酸链为引物进行pcr扩增，得到128bp的扩增产物。将得到的扩增产物克隆到质粒载体pmd - 18 - t上，筛选反向克隆，然后将其反向构建到植物表达载体pbinyxw的camv35s启动子和tmv增强子“ ”的下游，构建成反义表达载体pbinya 。并在对河套蜜瓜授粉受精生物学研究的基础上，通过花粉管通道法转化河套蜜瓜，共获76颗瓜，并进行了硬度和含糖量的分析。
• However , only did a few pollen germinate in inter - specific hybridization between cerasus fruticosa and c . avian and many pollen tubes are crosswise on stigma and couldn " t penetrated into style . although a few pollen tubes entered , they couldn " t continue to grow due to accumulation of callosity in pollen tubes . in view of above - mentioned results , the irregular growth of pollen was the main reason to cause the incompatibility of distant hybridization between cerasus fruticosa and c . avium
  而草原樱桃与欧洲甜樱桃种间杂交授粉后花粉在柱头上虽少量萌发，但花粉管在柱头上横向生长，或盘绕，扭曲不能伸入花柱；个别进入花柱的花粉管先端因沉积胼胝质而中途停止伸长未能进入子房到达胚珠，从而说明这种杂交授粉后花粉管的不正常行为是导致草原樱桃与欧洲甜樱桃远缘杂交不亲和的主要原因。
• Histochemical gus assay showed that the gus staining was observed only in the mature pollen and germination pollen tube . there is no detectable gus activity in other floral organs , leaves and stems . these results suggested that st901 is a novel pollen - specific gene and the 288bp promoter fragment ( - 297 ? xfrom the translation start codon atg ) is sufficient for pollen - specific expression and os - element regulatory of st901 promoter was possibly concentrated in the region - 297 to - 9
  通过对gus酶活性的组织化学定位分析，表明， st901基因启动子驱动gus基因特异地在成熟花粉和花粉管中表达， st901基因具有花粉表达特性；且288bp ( - 297至- 9 ) ( atg定为+ 1 )的启动子区段足以驱动gus基因在花粉中的特异表达， st901基因启动子的花粉顺式表达元件可能位于- 297至- 9之间。
• In this study , in order to examine the dynamics of tip [ ca2 + ] i together with the dynamics of tip - localized f - actin in vivo , a kind of double labeled material was constructed . the ca2 + and actin microfilaments of arabidopsis pollen tubes were labeled by cameleon and gfp - mtalin respectively
  本研究以拟南芥花粉管为材料，通过转基因技术，将分别标记ca ~ ( 2 + )和微丝的两种荧光蛋白cameleon与gfp - mtalin在花粉管中同时表达，实现活体花粉管中ca ~ ( 2 + )与微丝的同时标记。