The cdna expression library that contains no intron theoretically include all expressed genes and conserve resource genes permanently . lt can be used to find out and clone some genes which can express particular protein with modern molecular biological techniques , such as immunological screening , drug - prob screening , southern et al . lt is very important to study the life nature of plasmodium falciparum in molecular level . with the developments of these studies , the drug - resistant mechanism of the plasmodium falciparum and the genes of some specific medicine binding protein can be made well - known . at the same time , the researches will do good to explain the mechanism of some specific medicines in order to design and screen new anti - malaria drugs 建立cdna表达文库在一次永久保存基因资源的同时，可以利用功能筛选、免疫学筛选、药物探针筛选、 southern杂交和大规模序列测定等现代分子生物学技术寻找特异性活性蛋白基因，进而克隆和表达这些基因，对从核酸及蛋白质等分子水平研究疟原虫的生命活动规律，对揭示其抗药性分子机理，搞清某些特效药物结合蛋白的基因及此类药物的作用机制，对新型抗疟药物的合理设计及筛选都具有极其重要的现实意义。
Recently , a noval brain - specific na ^ - dependent inorganic phosphate transporters was successfully cloned using the method of molecular biology and was named dnpi because they had the characters of the vesicular glutamate transporter ( vglut ) . some studies showed that dnpi were distributed extensively in the brain area where glu was used as the neurotransmitter , such as thalamus , medulla oblongata and spinal cord . the ultrastructural studies indicated that most of dnpi are located in the presynaptic terminals and distributed on the synaptic vesicular membrane 最近人们应用分子生物学技术又成功地在哺乳类动物脑内克隆出一种新的脑内特殊的na ”依赖的无机磷酸转运体（ brain七pecificna dependentinorgarucphosphaternsporter ， bnpi ） ，并发现它也具有囊泡膜谷氨酸转运体（ vglllt ）的特点，因而被命名为dnpi （又称作vgiutz人新近的研究报道， dn ’ pi广泛地分布于运用m作为神经递质的脑区，如丘脑、延髓和脊髓。
The rapid development of modem molecular biology technique suppies a possible and efficient way for us to solove the problem completely with a comprehensive researches results to transgenic fish in recent years both domestic and aboard , it proves that it " s possible and makes great senses to cultivate a high quality fish to meet the satisifaction of the people 现代分子生物学技术突飞猛进的发展为我们切底解决这一难题提供了可能性和有效途径。综观国内外近些年来对转基因鱼的研究及结果证明，通过转基因定向培育出符合人们所需要的优良鱼种是完全可能的，也是十分有意义的。
In the meantime , congenital amblyopia resulted from optic nerve lesion was observed in the crested ibis , with an incidence rate of 3 - 5 % . since the epilepsy and congenital amblyopia are both hereditary , it is feasible to perform genetic engineering studies on the correlative genes , which could protect neurons from the damages of excitation toxin and vision sickness , in order to cure the diseases at gene level 由于癫痫和视神经病变类疾病均属于遗传性疾病，因此，利用分子生物学技术，对因癫痫和遗传性弱视症导致的神经元损伤具有保护及再生作用，以及视神经通路的发育、分化和受损视觉通路具有修复作用的相关基因，开展克隆、表达和活性鉴定之研究，将为通过基因工程技术实现其疾病治疗之目标奠定基础。
This paper discusses on some important progresses obtained in the field of life science such as the newly developed pharmaceutics making people rejuvenated , the first case that the mammals give birth to its own clonal baby , the reproductive biological techniques with the characteristics of ooplasm substitution , the research on the measurement of human genome sequence , the activation of melanocyte changing the white hairs into the black ones , and the research on rice cytology , etc 论述了使人变得年轻的制剂、世界第一例哺乳动物生下自己的克隆体、以卵浆置换为特征的生殖生物学技术、人类基因组测序研究、激活黑素细胞使白发变黑发以及水稻细胞学研究取得的重大进展,并阐述了其生物学意义和应用价值。
Many of them gave consent for partaking in the department s study by giving a small amount of blood , less than 3 ml . more than 1 , 000 specimens have been collected . advanced techniques of molecular biology by a systemic and comprehensive approach , including polymerize chain reactions and dna sequencing were applied for analysis of the samples 接受服务的病人中有许多同意抽取小量血液样本供该系作研究之用，该学系并已收集了超过1 , 000个血液样本，用先进的分子生物学技术进行系统性和详细基因研究。
All these could be improved by means of gene engineering . through the screening of phytase - producing mi crobial strains , cloning and modifying of phytase gene and constructing engineered strains by molecular biology methods , it is possible to increase the production of phytases , modify its properties , reduce costs and increase its efficiencies . presently it ' s one of the research hot points the worldwide 通过植酸酶产生菌的筛选和基因克隆，并利用分子生物学技术构建基因工程菌，或在分子水平上改造植酸酶基因，以提高植酸酶产量或改变植酸酶酶学性质，从而降低生产成本，提高其使用有效性，己成为世界性的研究热点之一。
Based on the previous studies , the research in this paper was carried out , mainly including two parts as follows : ( 1 ) anammox bacteria and aerobic ammonia oxidizers were detected in situ in 6 sediment samples taken from jiangsu province . molecular techniques , such as fish , pcr , dna cloning and sequencing etc . were used for this purpose . ( 2 ) the continuous cultivation of anammox bacteria from sediment samples were studied , which provides experimental basis for the bioaugamentation of eutrophicated sediment applying anammox process 本论文在前人研究的基础上，开展了以下两个方面的工作： ( 1 )采用分子生物学技术荧光原位杂交( fish ) 、多聚酶链式反应( pcr ) 、 dna克隆和测序等对采自江苏省苏州市、东太湖、新沂河等6个底质样品进行了厌氧氨氧化菌和传统氨氧化菌的原位检测； ( 2 )探讨了以底质作为接种体进行厌氧氨氧化菌富集培养的可行性，为天然底质环境中厌氧氨氧化过程的强化，富营养化底质微生物修复的可行性提供一定的依据。
In recent decades , extensive researches have been done and good progress have been made . many cytokine genes have been cloned and expressed . the genes of porcine il - 10 and il - 12 have been cloned and expressed in prokaryotic expression system and in eukaryotic expression system abroad , but no report is available in china 近十余年来，兽医上利用分子生物学技术对细胞因子进行了较多的研究，取得了重要进展，克隆出了一些细胞因子，猪il - 10 （ pil - 10 ）和猪il - 12 （ pil - 12 ）在国外已被克隆并被成功表达，而国内目前未见相关报道。
