Using the tpsl gene digested form the prokaryotic expressing vector as template , according to the published sequence , pcr primers of tpsl gene was designed , 1500bp fragment of tpsl was generated 以从t - vector中酶切得到的tps1为模板，根据已知序列设计pcr引物，扩增海藻糖- 6 -磷酸合成酶基因( tps1 ) 。
Keeping the ph at 6 . 0 , 3 % of the initial glucose concentration , 5 % of seed volume , 1 % of glucose concentration , heat shock at 50 ? for 6 hours resulted in 8 . 94g / l trehalose 研究还发现，选用5接种量， 3初糖浓度，反应过程中底物浓度1左右， ph6刀， 50 ’ c条件下震荡反应6h ，海藻糖合成量可达不94g l 。
The effects of six effectors , polyethylene glycol ( peg ) 6000 , d - sorbitol , trehalose , dithiothreitol , glycerol and glutarie dialdehyd on pal activity in enzymatic reaction were investigated in this article 摘要实验考察了聚乙二醇6000 、 d -山梨醇、海藻糖、二巯基苏糖醇、甘油、戊二醛对酶反应中苯丙氨酸解氨酶活力的影响。
By response surface experiment , it was found that the combination use of 0 . 54 % peg6000 , 0 . 62 % trehalose and 0 . 31 % dtt could effectively improve the pal activity by 92 . 9 % 响应面实验发现，海藻糖、聚乙二醇6000 、二巯基苏糖醇在实验浓度范围内交互作用很小， 0 . 62 %海藻糖、 0 . 54 %聚乙二醇6000和0 . 31 %巯基苏糖醇的组合可提高酶活92 . 9 % 。
When a protein is desiccated in the presence of trehalose , water molecules bound to the protein structure by hydrogen bonding evaporate and the sites are then replaced by the sugar to maintain an intact confirmation ( 42 ? 45 ) 当蛋白在海藻糖存在下干燥，经氢键结合在蛋白结构上的水分子蒸发掉，留下的位点随后被糖替代，确保了结构的完整。
In order to enhance the trehalose production , y7 was used for mutation with ems . through primary screening and compound screening , strain l61 with high - yielding trehalose and deep neutral trehalase activity was screened 实验对出发菌株y7进行了ems诱变处理，通过初筛、复筛，从中选育出一株能高产海藻糖同时具有较低海藻糖分解酶活性的菌株l61 。
Keeping the ph of the culture broth at 6 . 0 , the initial glucose concentration of l % , the strain could produce 14 . 5g / l trehalose after reaction at 50 for 6 hours , the production of trehalose increased by 62 . 2 % than that of flask - shaking culture 0 ，温度50c条件下，发酵6h ，可使海藻糖的合成总量达14 sg l ，比摇瓶反应的海藻糖合成量增加了62 2 。
The methods of paper chromatography - mottle elution cnromometry and enzyme - dns colorimetric were defined . these methods were simple , cheap and accuate . the results showed that the positive correlation was obtained between trehalose concentration and acetic acid or osmotic tolerant 提出了“纸层析-分离洗脱法” 、 “酶- dns比色法”定量测定海藻糖的方法，该方法简便、成本低、准确度高。
The main research contents include the methods for trehalose determination , the breeding of trehalose high - producing strain , the optimization of medium and culture conditions , the production of extracellular trehalose and the purification of the intra - and extracellular trehalose 研究内容主要包括海藻糖测定方法的研究、高产海藻糖菌株的选育、培养基与培养条件的优化、胞外海藻糖的合成以及海藻糖的提取与精制等。
