Pebe2 vector containing the target gene and 35sih3 vector containing bar gene were co - bombarded into several indica rice lines shuhui527 , minghui86 and 612s etc . resistant seedling were obtained finally . in order to establish rice mutation library , agrobacteriwn vector pshk22 containing bar gene as screening maker was transformed iniojaponica zhonghual 1 在水稻的遗传转化过程中，通过基因枪法将含有目的基因sbe2b的质粒载体pebe2和含有筛选标记基因bar的质粒载体35sih3共转化到蜀恢527 、明恢86 、 612s等籼稻品种中去。
4 . at the first time , we use camv35s and fmv promoter , nos terminater , mark gene nptii , and aim gene pat , epsps and cryia ( b ) genes as collate , design pairs of specific primers , screen the optimum primers groups , optimized the test condition and parameter , establishing the qualitative pcr detection system , the sensitivity is 0 4 、首次以camv35s 、 fmv启动子、 nos终止子、标记基因nptll 、抗除草剂基因epsps 、 pat 、抗虫基因cry1a枷）为检测目标，设计、筛选出特异性引物，优化实验参数，建立了转基因植物pcr定性检测方法体系，灵敏度达0
Methods : ( 1 ) the segregation of foreign target gene in the t1 by histochmical gus assays ; ( 2 ) identification of pure line from transgenic tomatoes ( tl ) through examining gus expression in pollens in conjunction with pcr analysis of marker gene ( npt ) ; ( 3 ) the transcript levels of leetrl or leetr2 in anti - sense transgenic plants ; ( 4 ) the phenotypes of the transgenic plants in tomato during whole life cycle under ethylene - treated and non - treated conditions 本研究以反义乙烯受体leetr1 ， leetr2基因番茄t _ 0代种子为实验材料，利用gus基因表达研究外源基因的遗传规律，并借助于pcr技术对目的和标记基因的鉴定获得转基因t _ 1代材料。利用gus基因在t1花粉中的表达鉴定获得转基因纯合植株。研究了转基因后代的生长发育模式、对外源乙烯敏感性，以及靶基因的表达特性，初步探明了它们在乙烯受体系统中的功能。
In order to form a chloroplast transformation system of d . salina , we have conducted some studies including its sensitivity to antibiotics , the activity of promoter , cloning of the chloroplast genes and construction of transformation vectors , so far a pilot transformation system of the d . salina chloroplast has been completed . methods : the sensitivity of d . salina to seven antibiotics or herbicide used commonly in gene engineering was studied and the biological activity of atpa promoter from c . reinhardtii chloroplast was tested by using enhanced green fluorescent protein ( egfp ) as a reporter . primers were designed in the conservative encoding regions according to the chloroplast genomes from four algae which have close genetic relationship with d . salina , and the sequences of 16s rrna , chll and chln of d . salina chloroplast were cloned and sequenced , respectively 方法:根据杜氏盐藻的近缘藻类的叶绿体基因组序列资料，在基因编码区的高度保守区域设计引物，克隆了杜氏盐藻叶绿体165识na基因、咖l基因和ch n基因，并分别以165识na基因和chln基因序列为同源片段，以cat和bar基因为筛选标记基因构建了三套杜氏盐藻叶绿体转化载体: 2郑州大学2003年博士学位论文杜氏盐藻( d ~ iiellasalina )叶绿体转化研究pds165一eaf 、 ptn1269一bar和psp72一5一bar一3 ，用基因枪法转化杜氏盐藻，初步建立起杜氏盐藻叶绿体转化体系。
