Two major hydrophilic peaks were conserved as well . however , there are two amino acid substitutions at positions 280 ( n to s ) and 290 ( m to l ) in the second minor hydrophilic peak of the strain zj2000 , which might have a critical effect on antigenecity Jd1株在决定其抗原性的大小两个亲水区内高度保守，而zj2000株第二个小亲水区内280位氨基酸由s替代了n 、 290位l替代了m ，这两个突变可能与zj2000株的抗原漂移和毒力变异有关。
For creating a reasonable displaying method , momp gene fragment and pbv221 are recombined oritationally they are transformed into e . coli jm109 ( de3 ) . a expressing protein was got with momp antigen . this study establishes the foundation for advance research of momp 设计一条较为合理的表达方案，将momp基因片段与pbv221定向重组转化大肠杆菌jm109 ( de _ 3 ) ，得到具有momp抗原性的表达蛋白，为研究momp的生物学功能以及研制沙眼衣原体基因工程亚单位疫苗奠定了基础。
Hemagglutinins inhibition ( hi ) testes showed that there were obvious variations among the isolates from different geographical areas . this result explained why the protection rates of current vaccine were different around the country . dna sequence analysis of h9 hemagglutinin genes showed that these viruses were closely related and possibly came from one source 且在同一地域内，不同的异变体之间的差异较小，随着时间的推移，其变异并不明显；但在不同的区域内分离的毒株之间变异较为突出，这表明中国大陆h9n2亚型禽流感病毒不同分离株抗原性是与地域的改变而发生较为明显的变异。
The mechanism of ki 11 ing the schistosomulae in vitro depending on the specific ige antibody were observed . the distribution of the protein encoded by the cloned gene was determined by immuno - electromicroscopy with protein a - colloidal gold . in order to further analyse the epitope features of the protein relevant to the specific ige antibody , the phage display library of random peptides was screened by pooled sera with the specific ige antibody 借助现行生物信息学分析手段，通过互联网进入genbank数据库，对sj43b与已知序列进行同源性比对，应用dnatoois软件对其编码蛋白序列的氨基酸纽成、亲水性、抗原性和可及性进行分析，并在blastp程序下在genbank蛋白数据库中对sj43b编码3人卞医科大学博士学杠论文蛋白进行同源性检索。
Meanwhile , the male mice deficient for germ - cell cyritestin are infertile . therefore , both spl7 and cyritestin are important molecules in the process of fertilization . nevertheless , people can not get fully satisfied the results in some contraceptive researches for their limited effects respectively 其n端含有去整合素结构域，然而多数实验结果表明单一应用这些分子所获得的免疫性避孕效应非常有限，从而提出今后的研究方向是更具抗原性或良好运载体系的新型多价疫苗。
1 . cloning of the gene encoding the specific ige antibody related antigen of schistosoma japonicum the serum samples from the inhabitants living in the epidemic regions of schistosomaisis japonica were collected and detected by abc - elisa method for their swap specific ige antibody . the sera with high titer of the specific ige levels were chosen and pooled 89 ，其亲水性、抗原性和可及性分布曲线基本一致，预测抗原表位分另ij在第25 35位、 75 82位、 1h 1们位、 117 1n位和149 159位氨基酸残基。
To elucidate the antigenic drift and evolution of h9n2 subtype avian influenza viruses ( aivs ) , five isolates from the north of henan province during 1998 - 2002 were compared and analysed by cross - hemagglutinin inhibition test ( hi ) , cross - virus neutralization test ( vn ) in the chicken embryo and chicken embryo primary kidney cell ( cek ) and cross protection against challenge infection test 为了探讨h _ 9n _ 2亚型禽流感病毒的抗原性有否发生漂移，本研究从生物学角度和ha基因分子水平上对1998 ? 2002年间在河南省豫北地区分离到的5株h _ 9n _ 2亚型禽流感病毒的抗原性变化进行了比较和分析。
A miniature swine model of nerve transplantation was used to investigate the effects of preoperative ultraviolet - b ( uv - b ) - irradiated donor alloantigen portal venous infusion and injection of cultured major histocompatibility complex ( mhc ) - matched schwann cells into the nerve graft 本试验通过一个小型的猪的神经移植模型来调查经以下处理后移植物的抗原性：术前紫外线- b照射供体移植物，肝门静脉浸泡神经移植物以及向移植物内注射培育过的主要组织相容性复合物配对雪旺细胞。
Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding - peptides , we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library , which may be another form of antagonist for tnfa , and the mimotopes were identified by sandwich elisa . after 3 rounds of screening , we got 9 phage clones identified as positive clones which can bind with mcab j1d9 . we also identified the binding between mimotopes and tnf receptor by competitive elisa , and the results showed strongly binding . the amino acid sequence results shown three different sequences : c - rrpaqsg - c - nkhnrki - c and c - rgmsrki - c 在对噬菌体环七肽库进行三轮亲和性筛选后，随机挑选20个噬菌体克隆， elisa鉴定出9个阳性克隆，经dna测序推出三种氨基酸序列： c - rrpaqsg - c 、 c - nkhnrki - c和c - rgmsrki - c ，其中优势克隆序列为c - rrpaqsg - c ；鉴定结果显示阳性克隆能够与tnf受体结合，并且能够阻断tnf与受体的结合，提示筛选得到的环七肽克隆展示肽具有tnf的抗原性及与tnf受体结第一军医大学顾士学位论文合的特性，为tnfa表位模拟肽。
A rabbit was infected with a cloned yntatl , blood was collecting from from the rabbit every 3 days after infection within 30 days , 10 clonal trypanosome populations were gotten , infecting a new rabbit by the last non - cloned trypanosome population . repeated above all , thus infected 5 rabbits sequentially . twenty different vats ( variant antigen type ) were monitored and characterized from those fifty mono - clonal populations by indirect immunofluorescence test ( ift ) and avidin biotin enzyme immunoassay ( abc - eia ) 用伊氏锥虫云南水牛单克隆株yntat1感染兔，感染后30天内，每3天从兔血中分离锥虫并单虫克隆，最后一个未单虫克隆的虫株感染另一只兔，重复以上操作，这样顺序感染5只兔子，共获得50个单克隆锥虫种群( tp ) ，经间接免疫荧光和abc酶标试验鉴定共为20个抗原性互不相同的抗原变异体( vats ) 。
