Prevention and cure of the derzsy ' s disease depended on vaccine and antiserum , antibodies of eggs . the vaccines includes goose embryo and duck embryo vaccines which were used 1n breed goose and goslings , and those vaccines have great effect in breeding goose , but the entire virion live vaccines and attenuated vaccines exist many deficiencies . such as preclinical infection , dissemination of virus , recovery of viru5 , etc . those proplems can be sol / ed by producing genetic engineering " asclne 目前使用的疫苗分为种鹅用和雏鹅用的鹅胚和鸭胚化疫苗，这些疫苗在实际生产中发挥巨大作用，因其为全毒苗或弱毒苗，存在着潜伏感染，排毒散毒，毒力返祖的缺陷，基因工程疫苗的研制可解决上述问题。
It was interested that there was an extra six nucleosides insertion between 1647 - 1652nt ( according to the genomic sequence of la sota strain ) , and the sequence were cccccc in f48e9 strain , and tcccac in zj1 strain . in order to test if insertion of this six nucleosides is related with the virulence of nd , two primers were designed to amplify the same fragment of another ten ndv strains . the result of sequence comparison of 16 strains showed that the six nucleoside was absent in lentogenic strain . this suggested that the six nucleosides insertion might have relationship with the ndv virulence . compared with all known sequence of ndv . there was a special sequence ( 5 ' tctctctcctctctcctcc3 " ) in the genomic cdna of ndv f48e9 strain 通过rt - pcr方法扩增获得了另外10个背景清楚毒株的np - p基因间隔区片段，将这些序列与f48e9 、 lasota 、 clone30 、 b1 、 zj1和v4的相应序列进行了比较，结果在参比的16个ndv毒株中在该区段中除了有多个点突变外，个别毒株有碱基插入和缺失，所有以lasota株为代表的弱毒株均无6碱基的插入，而以f48e9株为首的强毒株均有此6碱基的插入，但有一个中等毒力的毒株dp没有6碱基的插入，不过它的基因序列和lasota的几乎相同，对于所克隆到的基因的代表性还有待确定。
The study is mainly about finding the reason that caused atypical hc through analysis and homology comparison on e2 gene sequences and amino acid sequences of e2 glycoprotein among strains hclv , fc , hcv - jn , hcv - yc , shimen et . al . at last , the best program against hc was established in order to control and erase hc 本研究主要是对猪瘟兔化弱毒疫苗、猪瘟病毒野毒株抗原基因( e2 )进行序列分析和同源性比较，分析发生非典型性猪瘟的原因，并在此基础上制定最佳猪瘟免疫程序。
