基因表达谱

• Here i reviewed the previous work on the gene expression patterns of human brain and testis , and proposed some potential suggestions for future research
  本文对人睾丸和脑基因表达谱的研究进行了回顾，并提出了该研究方向今后的一些研究设想。
• Then cdnas were labeled with cy5 or cy3 respectively by incorporating cy5 - dctp or cy3 - dctp into the reaction during reverse transcription of rna
  采用cartesian基因芯片打印仪，将纯化的cdna片段打印在corning玻片表面，制作了酵母细胞基因表达谱芯片。
• Methods dna chip was used to detect the mrna from 11 human transitional cell carcinoma tissues and to investigate the genes related to signal transduction
  方法使用人肿瘤基因表达谱芯片检测11例膀胱移行细胞癌组织基因表达谱的变化，以寻找与细胞信号转导相关的差异表达基因。
• Based on the homology comparison , gene expression pattern and the domain analysis , we presumed that a - 1b glycoprotein might play a role in the proliferation process of the cell
  通过同源分析、基因表达谱分析及结构域分析，推测- 1b糖蛋白可能在细胞的增殖过程中起作用。
• 2 . 4 affymetrix moe430a genechip analysis of expression pattern differences of undifferentiated escs and neural induced cells . 2 . 5 telomeric repeat ampliation protocol ( trap ) assays
  2 . 4采用月升metrixmo以30a小鼠表达谱芯片进行未分化及分化后第4 、 10天细胞基因表达谱差异的分析。
• Therefore , it is important to study the profiles of gene expression , which will help understand the global cellular stress responses to chemical carcinogens , and further elucidate the mechanisms of nontargeted mutagenesis
  所以，从整体上研究烷化剂作用后细胞基因表达谱改变，对于了解化学致癌物诱发的哺乳动物细胞应激反应的全貌和揭示非定标性突变的发生机制具有非常重要的意义。
• The labeled cdnas were used to hybridized with the prepared microarray . by using the dna microarray scanner and analysed with the quantarray software package , gene expression profiles of s . cerevisiae in response to heat were studied preliminarily
  最后提取热休克前后酵母细胞的总rna ，经逆转录标记合成cdna后与酵母基因表达谱芯片杂交，初步研究了酵母细胞在热应激时基因表达的变化。
• Techniques of differential display for gene expression profile , recently developed , are effective and practicable to explore gene expression differences of cells under different conditions , and used in the fields of plant - insect interactions and searching insect - resistant genes successfully
  摘要基因表达谱差异显示技术是最近兴起的研究同种细胞在不同状态下基因表达差异的一种有效手段。
• Sh - sy5y cells were differentiated into neuron - like cells by ra treatment in low concentration , total rna was extracted and labeled to hybridize with the microarray , the changes on gene expression were detected to find differentially expressed genes
  首先应用低浓度的ra处理sh - sy5y细胞，促使细胞分化成神经元表型，然后提取总rna并进行标记，与制备的基因表达谱芯片进行杂交，检测基因表达变化，查找差异表达基因。
• Our results also demonstrated that microarray technology was a powerful tool in analyzing the gene expression profile of induced gene expression . our in - house made cdna microarray could be successfully applied to the analysis of high throughput and parallel analysis of hundreds of genes
  我们的实验，证明了基因芯片技术是研究基因表达的有效工具，同时也说明了我们构建的k562细胞基因芯片可以成功地用于基因表达谱的研究。