In order to understand the mechanism of mtx further and to investigate the genotoxic target organs , we studied the dna damage and the correlation with dose of mtx by using the alkaline single cell gel electrophoresis ( comet ) assay . liver , spleen , bone marrow , thymus , kidney , testicle , stomach and peripheral lymphocytes of mice were isolated at lh , 3h , 6h , 12h , 24h after 5mg / kg mtx intraperitoneal injection 为了进一步了解甲氨蝶呤( mtx )的作用机制，探测其作用的遗传毒性靶器官，为应用mtx治疗过程中的临床监测和副作用防治提供理论依据，我们以小鼠为研究对象，用单细胞凝胶电泳技术检测了mtx腹腔注射染毒1h 、 3h 、 6h 、 12h 、 24h后对肝、脾、骨髓、胸腺、肾、睾丸、胃和外周血淋巴细胞的dna损伤作用及损伤程度与mtx剂量间的关系。
