In this paper , the basic thought of enterprise resource planning ( erp ) and the structure of java 2 platform enterprise edition ( j2ee ) are introduced . combined with a practical scientific research item : yz - erp for yueyang paper industry group , it summarizes the management actuality of yueyang paper industry group , requirements and conceptual design , and puts forward an solution of yz - erp based on the j2ee in b / s mode and the realization of yz - erp with some related technique 本论文介绍了企业资源计划( erp )的基本思想以及java2平台企业版( j2ee )体系架构，结合实际科研项目： 《岳阳纸业股份集团erp系统( yz - erp ) 》 ，在总结了岳阳纸业股份集团管理现状、需求和总体设计的基础上，提出了基于j2ee体系结构b s模式的yz - erp系统总体解决方案，并采用相关技术开发和实现了yz - erp系统。
The engineered strain ghd - yz26 / e . coli bl21 ( de3 ) was incubated and induced at 30 for 8 hrs . the results show that the target fusion protein is soluble and active , which is about 15 % compared with the total proteins in cells , and the enzymatic activity reaches 5 u / ml that is about 8 folds compared with the activity of strain yz - 26 Colibl21 ( de3 )中30诱导培养8hrs ，可获得可溶性表达，融合蛋白的表达量约占总菌蛋白的15 ，其酶活力为5u ml ，约是原始菌株的d -海因酶表观活性的8倍。
As far as the enzymatic activity is concerned , on the one hand , strain yz - ii6 has higher d - hydantoinase activity and lower d - carbamoylase activity so as not to be suitable for one - step bioconversion of d - amino acids , on the other hand , the higher hydantoinase activity , engineered strain in particular , may be convenient to be as a biocatalyst to produce n - carbamyl d - amino acids which hard to find in the markets Yz - 6菌具有较高的海因酶活性，但n -氨甲酰基d -氨基酸酰氨水解酶活性很低，仍不适合用于d -型氨基酸生产工艺中的一步法转化。另一方面，含海因酶基因的人工菌株不但酶活性高，也排除了天然菌株转化生产d -型氨基酸过程中的一些副产物。
The engineered strain ehd - yz26 / e . coli bl21 ( de3 ) was incubated at 30 for 10 hrs without adding any inducers . the results show that the target product is soluble and active , which account for about 20 % compared with the total proteins in cells , and the enzymatic activity reaches 13 u / ml that is about 13 folds compared with the activity of strain yz - 26 Colibl21 ( de3 )中30生长10hrs ，在不需添加任何诱导剂的情况下可获得可溶性表达，目的蛋白的表达量约占总菌蛋白的20 ，其酶活力为13u ml ，约是原始菌株的d -海因酶表观活性的13倍。
The advantage is that in view of the actual mobile telecom market of yz city during the research we comprehensively adopt many scientific market research methods such as questionnaire , data analysis method , depth interviews and subjective probability then use the spss to analyze the data to get plenty of valuable market information . the advice we offered can be profitable strategic reference when the mobile telecom company set down their marketing policies on market development and product development 突出的地方在于结合yz市移动通信市场实际情况，科学地采用了分层抽样方式，综合运用了问卷法、资料分析法、主观概率法和深度访谈法等调查方法，使用了spss软件进行统计分析，从而获得了丰富的较有价值的市场信息，并从市场基本战略的角度提出针对性的策略建议，为运营商进行市场和产品开发及市场战略的制定提供有益的参考。
The recombinant harboring the enzyme gene was transferred into e . coli bl21 and the engineered strain was expressed in lb medium by the induction of iptg . the results point out that not only the target protein can be expressed in soluble form , but also the bioactivity increase 6 times compared with that of strain yz - ii6 重组子pet3a - hdtase在大肠杆菌bl21中培养并用iptg (终浓度为1mm )诱导，海因酶不但获得可溶性表达，而且酶活可达4 . 08u ml ，比原始菌株yz - 6的海因酶活性提高了约6倍，最佳表达条件正在进一步实验中。
