When the time prolonged for 10 ~ 20min , the blastocysts rates declined to 47 . 62 % ~ 0 % . the results of cryopreservation of mouse 2 - cell embryos by straw and ops vitrification method in this study showed that when the 2 - cell embryos were equilibrated in efs40 at 20 for lmin ( one - step straw method ) before cryopreservation , the blastocysts rate was only 35 . 00 % . there was a significant difference ( p < 0 . 01 ) from the control ( 65 . 00 % ) 小鼠2 -细胞细管法和ops法玻璃化冷冻保存试验表明：在20室温条件下，用efs40平衡1min细管一步法冷冻?解冻后，经培养其囊胚发育率仅为35 . 00 ，和新鲜2 -细胞胚胎体外培养的对照组( 65 . 00 )的差异极显著( p 0 . 01 ) 。
It is financial institution to more suit development of modern economy , because it characterizes own balance sheet , internal governance structures , as well as subdivision of investor and vitrification of work mechanism etc . based on the historic analysis about investment , the concept of the investment fund institution was definitely put forward 它以其特有的资产负债结构、内部治理结构，以及投资者细分和透明化运行机制等特征，成为一种更适应现代经济发展的金融制度。在对投资基金进行历史分析的基础上，笔者明确地提出了投资基金制度的概念，之后运用相关的经济学原理和金融理论，对这种新型金融制度进行了系统的学理解析。
Based on how to scientifically and effectively designthe instruction , this study , combing educational psychology ' s cognitive instructional theory and constructivist instructional theory , suggests that instruction design must carry through vitrification instruction strategy , which means to open what was supposed to be the teachers ' matter in the traditional instruction theory , such as instruction object instruction preparation , instruction intention , instruction method and so on 摘要本文从如何科学、有效的对教学进行教学设计的角度出发，结合教育心理学的认知教学观和建构主义教学观，提出教学设计要贯穿“透明化”教学策略。
Get 4 right anterolateral femoral flaps and 4 left scapular flaps which were dyed obviously after 3 days , dehydrate by graded ethanol , vitrification and paraffin embeded , horizontally and vertically sliced continuously , thickness is 15 , 20 and loojtim respectively , he dyed , observed under microscope . results : ( 1 ) direct cutaneous artery or musculo - cutaneous artery , in the procedure of common integument tissue being shallowed up step by step , continuously sending out branches to deep fascia layer , superficial fascia layer , hypodermis layer , hypopapilla layer and papilla layer to form the five - grade cutaneous microcirculation " blood vessel tree " of the skin blood vessel network that can be identified 结果门）亘接皮动脉或肌皮动脉，在体被组织逐步浅出的整个行程中，不断发出分支并分别在深筋膜层、皮下组织（浅筋膜）层、真皮下层、乳头下层和乳头层内形成五级具有鉴别特征的皮肤微循环血管网（血管树） 。 （ 2 ）体被组织静脉血管网由浅入深分为五层：皮肤乳头层。乳头下层、真皮下层、皮下组织层和深筋膜层。
The phenomenon of vitrification phenomenon is a big obstruction in the propagantion of carnation by tissue culture , the effective way to decrease the vitrification phenomenon is to increase the concentration of sucrose and agar in the medium , to use strong luminosity of 10000 - 20000 lx , and to decrease the concentration of hormone 摘要香石竹试管苗玻璃化现象给试管苗的应用带来困难，采用强光照10000 - 20000lx ，在培养基中提高蔗糖和琼脂浓度，降低激素用量，对克服香石竹试管苗玻璃化有明显效果。
This paper summarizes the research progress in vitrified cryopreservation of several animal tissues and cells , for example , pancreatic islet and oocytes of mouse , ovaries of rat , blastocysts and oocytes of bovine , embryos of pig and human , and the effect of different cryoprotectants , vitrification solutions and cooling procedures on survival rate 本文论述几种主要组织细胞玻璃化冻存方法的研究及其进展，如小鼠胰岛和卵巢、牛胚泡、牛卵母细胞、猪胚胎、人胚胎等，不同冷冻保护剂，玻璃化溶液和冷冻程序对细胞存活率的影响。
The mouse morulae were cryopreserved by vitrification in ops ( experiment 5 ) or straw ( experiment 6 ) followed by splitting . the fresh embryos undergoing the same procedure served as controls the rate of survival demi - embryos after splitting and the rate of demi - embryos developed to blastocysts in experiment 5 and 6 were similar ( p > 0 . 05 ) to those in controls , but the blastocyst rate in controls ( 77 . 38 % ) was a little higher than that in experiment 5 and 6 ( 70 . 22 % and 68 . 52 % ) 对细管法、 ops法冷冻后的胚胎进行分割，二者分割成功率、半胚培养后的囊胚发育率与新鲜对照组无显著性差异( p 0 . 05 ) ，但鲜半胚的囊胚发育率( 77 . 38 )略高于细管法( 68 . 52 )与ops法( 70 . 22 ) 。
This paper has analyzed water activity of food and close relation with moisture content , temperature , vitrification transfer temperature , provided measure methods based on different principles , listed some measure apparatus in common use , appointed to the problem existing in measure methods and apparatus in use 摘要本文分析食品中水分活度，以及与含湿量、温度、玻璃化转化温度存在的密切关系；给出基于不同原理的测量方法，列举一些常用的测量仪器；指出现有测量方法、仪器待解决的问题。
By comparison of the four methods : simple freezing of proliferating meristems , vitrification of proliferating meristems , vitrification of isolated meristems , and droplet vitrification of isolated meristems for cryopreservation of meristems in banana , it is concluded that the droplet vitrification might be the best applicable protocol to " musa " germplasm 并分析了当前应用在香蕉分生组织上的4种超低温保存方法的优缺点，分别为：分生组织团简单冻存法、分生组织团玻璃化法、单个分生组织玻璃化法、滴冻玻璃化法，认为滴冻玻璃化法效果最好。
