By means of discontinuous tris - hcl buffer system and poly aery 1 amide gel electrophoresis ( page ) , zymogram of 23 species of 11 genera in 4 subfamilies mirinae , orthotylinae , phylinae and deraeocorinae are gained . by specific primer pcr and sequencing , 57 cyt b gene 433bp sequences are obtained from 34 species respectively belonging to 17 genera in 5 subfamilies mirinae , orthotylinae , phylinae , deraeocorinae , bryocorinae from 64 species of 23 genera which involved in this research , and 2 outgroup species of family anthocoridae as well . 1 首次通过特异引物扩增和基因测序的研究方法从被试的5亚科23个属的64种盲蝽中获得了盲蝽亚科、合垫盲蝽亚科、叶盲蝽亚科、赤爪盲蝽亚科和蕨盲蝽亚科bryocorinae5个亚科17个属的34种盲蝽以及外群花蝽科anthocoridae2种花蝽的cytb基因序列57条。
The obtained biosensor exhibits high sensitivity , excellent reproductivity and good stability with substantially improved performance . part two describes the manufacture and characterization of glucose oxidase - silver sol - polyvinyl butyral modified platinum electrodes with tris ( 2 , 2 ' - bipyridyl ) cobalt ( iii ) perchlorate as an electron transfer mediator in the glucose solution 用纳米ag溶胶固定god ，采用聚乙烯醇缩丁醛为辅助固酶膜基质修饰铂丝电极，并以葡萄糖溶液中的co恤pyh （ cio4 ） 3为电子媒介体组成葡萄糖氧化酶生物传感器。
Methods and results : proteomics approaches involve three critical techniques : two dimensional electrophoresis , biological mass spectrometry and bioinformatics . in 2 - de , we applied traditional ief in the first dimension and tris - tricine system in the second dimension and acquired protein profiles of serum and skin samples . in the identification of 10 proteins of serum and 16 proteins of skin , we acquired peptide mass of fingerprint ( pmf ) maps for all targets by means of matrix - assisted laser desorption - ionization time of flight mass spectrometry ( maldi - tof - ms ) and partial aminio acid sequences for 9 proteins by means of electrospray ionization ms / ms ( lisi - ms / ms ) 研究方法：采用双向电泳技术获取泥鳅创伤前后血清和皮肤的小分子蛋白图谱，利用基质辅助激光解析飞行时间检测质谱分析( matrix - assistedlaserdesorption - ionizationtimeofflightmassspectrometry , maldi - tof - ms )和电喷雾离子化串连质谱分析( electrosprayionizationms / ms , esi - ms hs )分别获得差异蛋白点的肽指纹图谱( peptidemassoffingerprint , pmf )和部分序列信息，通过互联网上的expasy服务器中和ncbi的相关软件将这些信息和swissprot数据库进行匹配鉴定蛋白质种类。
The wells of elisa plate were coated with pab ( 100ng / l ) against h3n2 , then phage was added to the wells . after incubation , the wells were washed vigorously with tbst to remove nonbinding phage . phage bound to the antibody were eluted with 0 . 2mol / l glycine - hcl ( ph2 . 2 ) for 10 min at room temperature and neutrialized with 2mol / l tris - hcl ( ph9 . 1 ) 以抗h3n2流感病毒的多克隆抗体( 100ng l )包被酶标板，加入制备好的肽库，用tbst洗去非特异结合的噬菌体，加0 . 2mol l甘氨酸-盐酸( ph2 . 2 ) ，室温放置10min以洗脱特异结合的噬菌体， 2mol ltris - hcl ( ph9 . 1 )中和后，取2 l噬菌体接种大肠杆菌xl1 - blue菌进行空斑滴定，其余噬菌体扩增后用于下一轮筛选，共重复3轮淘洗。
After centrifugation at 8000rpm for 15 minutes , the bacterial pellets was subjected to ultr asonication in the suspension solution ( 50mmol / l , ph8 . 0 tris - hci , 100mmol / l nac1 , 0 . 5mmol / l edta , 4mol / l urea , 0 . 2 % triton x - 100 , 0 . 2 % doc ) at 300w , working 3s , intervul 3s for 240 times . then , concentration at 8000rpm for 15 minutes at 4 : 3 ) p (正x一们’一1一nk4表达菌株blzi诱导持续时间的研究分别进行了1 、 2 、 3 、 4 、 5小时的诱导，发现在诱导2小时后表山西医科大学2003届硕士学位论文达即可达到最人。
At first , 1 . 67 u g per well mcab all was coated on three wells of a plate , and then 1 . 5 x 1011 phage virion was diluted and added , after incubating with the target , wash away unbound phage by tbst ( 0 . 1 % tween - 20 ) , the bound phage was eluted with ph 2 . 2 tris - gly buffer and amplified , the specially bound phage was enriched by taking through addition binding / amplification cycles . ln the following cycles , the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all , collecting and titering the washing phage of last time and output phage in each round , the selective ratio and the false positive rate of each round were worked out , the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective . after 4 rounds of panning , 11 phage clones were selected after competitive - ellsa , the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced , a clear consensus binding sequence emerged 在本实验中，利用随机12肽库对抗猪瘟病毒( classicalswinefeverviruscsfv )糖蛋白me2的单抗a11进行表位筛选，经过四轮筛选以后，随机挑取11个克隆作竞争- elisa检测，结果表明，所挑11个克隆中，有9个克隆能对me2蛋白和a11反应产生抑制作用，抑制率最高可达64 ； dna测序以后经过dnastar软件分析，发现它们的核心序列为anwralsl ，该核心序列与猪瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夹心- elisa检测和western - blotting试验均证明所挑阳性克隆能被a11所识别；人工合成含核心序列的多肽经间接elisa试验证实，也能被a11识别。
We used gradient dilution renaluration ( renaturation buffer : 0 . 14mol / l tris - hcl ph8 . 0 . 10mmol / lgsh , 2mmol / lgssg . 5 % glycerol , 0 . 02mol / l l - arg ) and gel filter renaturation by sephacral s200 to refold the inclusion body respectively . to get pure nk4 , it was purified by gst affinity gromatochraphy . to detect the angiostatic activity of nk4 in vivo , it was tested using cam 6 )诱导温度对pg厂x一4t一l一nk4表达菌株bl21表达目的蛋自的影响分别进行了25 ’ c 、 300c 、 37温度状态卜，以iptg化学诱导，发现其均可表达，全菌表达量: 37最高，其次为30诱导时。
The organosilicone monomer - [ tris ( trimethylsiloxy ) silyl ] propyl methacrylate was synthesized by trimethylchlorosilane ( tmcs ) and - ( trimethyloxysilyl ) propyl methacrylate ( kh - 570 ) , a improved synthetical method of organosilicone monomer tris with higher silicone content than raw monomer - ( trimethyloxysilyl ) propyl methacrylate ( kh - 570 ) was raised , and poly - - [ tris ( trimethylsiloxy ) silyl ] propyl methacrylate latex was prepared by emulsion polymerization , ir was used to study the polymer structure 然后，以此单体为原料用乳液聚合法制备了有机硅均聚物( ptris )乳液、有机硅丙烯酸酯共聚( p ( mma - co - tris ) )乳液和聚甲基丙烯酸甲酯( pmma )乳液。
The response of the glucose electrode is surveyed by determining cyclic voltammetric peak current values under aerobic solution conditions . electrocatalytic oxidation of glucose at the electrode is evaluated with respect to temperature , ph value of solution and concentration of tris ( 2 , 2 ' - bipyridyl ) cobalt ( iii ) perchlorate 实验用循环伏安法测定有氧溶液条件下葡萄糖在该生物传感器上产生的催化氧化峰电流，考察了温度、溶液ph值和co （ bpb浓度对传感器的电流响应性能的影响。
Western blot analysis we treated 200 fertilized eggs in a series of steps including lysing them in l0ul of lysis buffer ( 50mm tris - hcl ph 7 . 5 , 250mm nacl , 5mm edta , imm dtt , 0 . 1 % triton , 50mm sodium orthovanadate , looug / mg pmsf and tpck , 50ug / ml tlck , 1 ug / ml leupeptin pepstatin and aprotinin ) , frozing them below - 70 , and then thawing them at room temperature for 10 min 2 . westem印迹将处理组及对照组小鼠一细胞g2期受精卵各200个取出，转移到ep - pendoff管中， 5000甲m离心4分钟，弃去上清，加入ro川粉碎缓冲液，充分振荡混匀，在液氮中经过2一3次的冻融循环，迫使卵细胞裂解，加人等量的样品缓冲液沸水中煮5分钟，用于westem印迹分析。
