Ssmapkk showed highest homology to the npk2 gene from nicotiana tabacum . genomic southern blot analysis suggested there was only one copy ssmapkk gene in suaeda salsa genome Ssmapkk与已报道的烟草npk2同源性最高， southern杂交表明该基因在盐地碱蓬基因组中只有一个拷贝。
3 . southern blot analysis of pcr positive plants indicated that 3 plants were transgenic plants and the thymosin gene has been transformed into genome of lettuce . 4 Ctab法提取pcr阳性株的基因组dna进行southern杂交，其中3株为阳性，表明胸腺肽基因已经整合到生菜基因组中。
3 . twenty gus - positive tobacco lines were obtained by agrobacterium tumefaciens - mediated transformation of leaf - discs , and were confirmed by pcr and southern blotting analyses 将所构建表达载体通过农杆菌介导法导入烟草，获得20余株gus阳性苗，经pcr和southern杂交检测均呈阳性。
Southern blotting indicated that the pa7 fragment was present in p . pseudoalcaligenes genome probably as one copy . dot blotting indicated that the pa4 , pa7 , pas , pa9 were highly homologous 对pa7片段进行southern杂交鉴定证明该片段来自于类产碱假单胞菌基因组且可能以单拷贝存在。
Osdd2 gene existed as a single copy gene in the rice genome based on the result of southern blot analysis . we took a pcr method to clone the cdna of osdd2 gene 使用克隆的边端插入序列为探针对水稻(中花11品种)进行southern杂交分析，表明osdd2基因在水稻基因组中是以单拷贝存在。
Southern blot analysis suggest that s2 gene has been integrated in the genome of three pcr - positive regenerated plants . the rest two regenerated plants did not show a band corresponding to s2 取这3株的嫩叶，对照植株( 2株)的嫩叶，作hplc分析， 3株转基因植株中均检测到白藜芦醇的存在。
7 as indicated myb - like genes are members of families of related genes . we therefore performed a southern blot experiment to determine the approximate copy number of the mxmybl gene 7 、虽然myb基因是一个转录因于大家族，但我们通过soutnern杂交验证： mxmyb基因在小金海棠基因组dna中是单拷贝。
Cspds gene was successfully introduced into tobacco plant by agrobacteriwn - mediated ( agrobacterium tumefaciens eha105 ) transformation , and the transgenic plants were obtained and confirmed by pcr and pcr southern blot analysis 以密蒙花芽、幼叶、茎段为外植体，在b5 、 ms 、 wh1te培养基上获得愈伤组织。
Pcr ( polymerase chain reaction ) , southern blot analysis performed toward 10 transgenic rice plants revealed that foreign genes were integrated into the genome of these plants 选取部分植株进行pcr分析、 dna点杂交、 pcr southern杂交。以上分子检测结果表明，绝大部分经抗性筛选得到的转化植株的基因组中已整合有外源基因。
The transgenic goldfish were detected and screened by pcr , southern blot . the results showed that pcr amplify a 478bp specific molecular band in 5 individuals , southern blot results showed a strong signal in transgenic fish Pcr检测结果显示，有5尾转化个体的总dna能扩增出一条478bp的特异性分子带，说明转化个体整合有gfp基因，整合率为42 。
