Two image mapping algorithms of the volume rendering , forward mapping and backward mapping are researched on the basis of optical model for volume rendering . combined with the characteristic of a series of section images from laser confocal scanning microscopy , the volume buffer algorithm and maximizing algorithms are proposed in this paper . 4 同时在探讨体绘制光学模型的基础上，研究了体绘制的两种图像合成算法，即由前向后算法和由后向前算法，并结合激光共聚焦扫描显微成像系统序列断层荧光图像的特点提出了体缓冲器算法和最大值算法。
Single mode optical fiber laser confocal scanning microscopy is a new technology in microstructure studying . the speciality of the system is limiting the out - of - focus light reflected from out - of - focus plane in object to restrict the blur of image . therefore , the plane resolution and axial resolution of the system could be improved to sub - micron grade 单模光纤激光共焦扫描显微成像系统是研究微观结构的一种新技术，其本质是抑制目标物体离焦面所反射的光线进入系统，从而抑制这些离焦光线造成像点弥散斑增大的影响，使系统的横向与轴向分辨率得到大幅度的提高，能实现亚微米级的层析。
Fluorescence power transfer function , three - dimensional point spread function ( 3d - psf ) and three - dimensional optical transfer function ( sd - otf ) for the various fluorescent wavelength of the two kinds of fluorescence confocal scanning microscopy are calculated in this paper by using fourier imaging theory . the results show that the fluorescent wavelength has influence on imaging property of confocal microscopy such as spatial cut - off frequency , resolution and 3d - otf . there is a different missing - cone in the 3 - d space of otf when the ratio of excitation wavelength to fluorescent wavelength decreases 本文在sheppard和gumin等人的理论基础上，利用fourier光学成像理论，讨论了不同荧光波长对单光子和双光子共焦显微镜成像特性的影响，导出了单光子和双光子共焦显微镜的荧光功率传输函数、三维脉冲响应函数和三维光学传递函数，得到了它们在不同激发波长与荧光波长比值时具体的表达式，并且通过数值计算，得到了它们的曲线图，结果表明：随着激发波长与荧光波长比值的增加，焦斑的横向分布和纵向分布变窄，横向分辨率和纵向分辨率提高，系统的成像效果变好，当激发波长与荧光波长的比值下降到一定程度时，可以看到不同程度的失锥现象。
