When cultured in liquid medium , the growth period of a . ficoidea cv . " ruliginosa " hairy roots could be divided into three phases : lag phase , rapid - growth phase and plateau phase 而在添加6 - ba2 . 0mg l和naa0 . 8mg l的ms培养基上，部分愈伤组织能分化出幼芽。红龙草毛状根液体培养的生长过程分为三个时期：生长迟滞期、快速生长期、生长平台期。
More interesting , the content of secondary metabolite always increased markedly in hairy root . so the transgenic system by ri plasmid would be wildly used in plant breeding , plant gene engineering and pharmaceutical element production 发根农杆菌遗传转化体系的这一特点在作物育种、植物基因工程、次生代谢物工厂化生产上有广阔的应用前景。
Changes in contents of sucrose , nitrogen and phosphate in liquid medium were determined during the whole culture of hairy roots . the results showed that the consumption rate of sucrose in the medium was positively related to the growth rate of hairy roots 对毛状根液体培养过程中培养基内蔗糖、硝态氮、氨态氮以及磷酸盐含量的分析结果表明：培养基中蔗糖的消耗速率与毛状根的生长曲线呈正相关。
These hairy roots grew well on ms agar medium or liquid medium withour growth regulator for over one year . they grew vigorously and produced numerous lateral branches . four hairy root clones , namely tra1 , tra2 , tra3 and tra4 , were established 毛状根能在不含任何激素的ms固液体培养基中快速生长，且表现出多分支，多根毛，失去向地性生长等典型毛状根特性；建立了tra1 , tra2 , tra3 , tra4四个毛状根无性系。
Adventitious shoots could be rooted and formed regenerated plants on 1 / 2 ms medium supplemented with naa0 . 4mg / l . hairy roots could be induced from the basal surface of the explants 8 days after inoculation of a . ficoidea cv . " ruliginosa " leaf explants with a . rhizogenes atcc 15834 红龙草叶片外植体被发根农杆菌atcc15834感染8d后，从形态学下端叶脉处陆续分化出乳白色的不定根， 21d后，叶片外植体的生根率高达92 . 5 。
( 3 ) regenerating plantlets were obtained via somatic embryo , which derived from hairy root . the effects on the induction of somatic embryos from hairy root were investigated using orthogonal test . the best somatic embryo induction was obtained on ms + 0 . 5mg / l naa + 0 . 5mg / l kt + 30g / l sucrose 单独使用naa很难诱导出体胚，单独使用kt或kt与naa配合使用均能诱导出体胚， naa 、 kt和蔗糖对体胚诱导的影响作用依次是t a蔗糖。
The purpose of this study is to provide theoretical and technical support for torenia transgenic hairy root and other molecular researches used by torenia . the comparation and identification of agrobacterium rhizogenes strains were investigated using 3 - ketolactose test , the differential acid production assay and the motility assay 采用3 -酮乳糖产物法、差异酸生成实验和游动性实验鉴定6种发根农杆菌供试菌株a4 、 r1205 、 r1000 、 r1601 、 r1022和15834 。
Presently , the scientist have made out some successes by using biotechnology and molecuology , for example , planting out regeneration shoots , culture suspended cell in ferment tank , screening mutant tissue with high content of flavonoids , cloning and sup - expression of key enzyme , restraining derivative pathway by anti - dna or anti - rna and inducing genetic transformated hairy root 通过培养水母雪莲的发状根将是获得雪莲类黄酮极有前途的方法，目前还是一项待填补的空白。发根农杆菌( agrobacteriumrhizogenes )是一种革兰氏阴性土壤细菌，细胞内有200kb左右的双链闭环dna ? ri质粒( rootinducingplasmid ) 。
Tissue culture and plantlet regeneration from leaf explants of a . ficoidea cv . " ruliginosa " and its genetic transformation with agrobacterium rhizogenes atcc 15834 were investigated as well as changes in the contents of sucrose , nitrogen and phosphate in liquid medium during the hairy root cultures 本文研究了红龙草( altemantheraficoideacv " ruliginosa " )叶片的组织培养、发根农杆菌( agrobacteriumrhizogenes ) atcc15834对红龙草的遗传转化以及毛状根液体培养过程中培养液某些营养成分的消耗变化。
