The homology of recombinant virus bmpak - hbmp was obtained and identified by plaque assay and baculovirus contains the hbmp gene was confirmed through pcr and dna dot blotting . the expressed rhbsag was determined by elisa after infecting bm - n cells and pupae with recombinant virus bmpak - hbmp and bmpak - hbm ( containing nonfusion hbv surface antigen medium sized ) 用重组病毒bmpak - hbmp和bmpak - hbm [带有非融合乙肝表面抗原( pres2 + s )基因，为本实验构建]感染家蚕细胞及蛹，对两种病毒的表达产物用elisa进行了跟踪检测，结果表明，感染bmpak - hbmp的家蚕细胞及蛹中rhbsag的表达量分别为3
Expression analysis the expression analysis of gharf in different tissues of cotton was carried out by rna dot blotting with ghakf probe labeled with dig . the result showed that the expression of arf gene was mainly in anthers of sterile and fertile , pollen of fertile , corolla rather than in leaves , radicel and ovule 棉花ari ； ’基因的表达分析用地高辛将棉花arf基因标记为探针，与棉花洞a的不育株花药、司有株花药、可育花粉粒、叶片、花冠、胚根和胚珠的总rna进行斑点杂交。
This time , using cdna of zmcdc5 as template , we amplify a sequence by means of pcr technology . and then , using restrict endoenzyme and ligase , we conjunct the 0 . 8kb length dna sequence in a expression vector , pet - 30a . after induction , expression and purification , we obtained a 35 . 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ) . with the purified protein , we got its antibody and testified the antibody by means of western blotting and dot blotting 本实验是以zmcdc5的cdna为模板，使用pcr获得基因片段，再通过酶切连接，将得到的0 . 8kb的基因片段构建于pet - 30a表达载体上，经过诱导表达和纯化，获得zmcdc5的融合蛋白，其中包括了zmcdc5925个氨基酸中647 914共267个氨基酸残基
The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii . two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium . the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome . at the same time ; compared agrabactenum - mediated method with gene gun method , the transformation frequency of the former was 16 . 7 % , while the latter was 50 % , so gene gun transformation method was suitable for long ya liiliwn 用携带有几丁质酶基因和- 1 、 3葡聚糖酶基因的工程菌，通过农杆菌介导法和基因枪转化法转化龙牙百合，经pcr和点杂交检测证明外源基因已经整合到植物染色体中。同时对农杆菌介导法和基因枪法进行比较，发现农杆菌介导法的转化率为16 . 7 ，基因枪法的转化率为50 ，因此可能基因枪转化法更适于龙牙百合的遗传转化。
Was multiplied and the tk gene was cloned . the cloned tk gene was retrieved by proper restrictive hemodynamics . the retrieved tk gene was labeled by digoxin according to the kit of labeling and detection of digoxin . then , the specificity and sensitivity of tk gene probe were detected with dot blot hybridization . the sequence of tk gene of nm98a strain was analysed . the result of the analysis of tk gene ' s sequence confirmed that autoploidy between tk gene of nm - 98a strain and issued strain was 99 . 7 % 本研究中首次对iltv - nm98a株的tk基因进行了克隆和序列分析，结果表明： iltv - nm98a株tk基因的核苷酸序列与已发表的iltvtk基因的核苷酸序列具有高度的同源性，两者之间仅相差4个核苷酸，同源性高达99 . 7 ，从而证实了iltvtk基因是高度保守的，为iltvtk基因核酸探针的制备提供了有力的依据。
The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p . pastoris and transformed into p . pastoris by electroporation after linearization , 25 high - copied transformants were obtained by g418 screening . it was proved that the e2 genes were integrated stably into chromosome of p . pastoris by dot blot and dna sequencing 猪瘟病毒e2基因的真核表达：分别将csfv两个代表株的e2基因克隆入毕赤酵母( p . pastoris )分泌型表达载体ppic9k中，酶切线型化后电穿孔导入p . pastotis进行整合，经g418筛选得到25个高拷贝转化子，经dna斑点试验和dna测序证明外源基因e2稳定地整合到p . pastoris染色体中。
Angiostatin ( kl - 3 ) gene was inserted into bombyx mori baculovims transfer vector pbacpaks and cotransfected with lineared dna of bm - bacpak6 virus into bmn cells . the homologous recombination occurred inside the cells , and the recombinant virus bacpak - angiostatin was expressed , as identified by pcr and dna dot blotting 本研究首先将人血管抑素( angiostatin )基因重组于家蚕杆状病毒转移载体pbacpak8中，获得重组转移载体pbacpak - angiostatin ，并与线性化病毒bm - bacpak6dna共转染家蚕细胞株，获得重组病毒bacpak - angiostafin 。
