In this article , the highly - efficient plant vectors with bivalent genes have been constructed by combining the gene of vhb with bt gene and cfmcryla - cpti gene respectively , studying the influence over the plants metabolic level and growth . we manage to reach the goal of increasing the yield of transgenic plants and cultivate new plant varities with pest - resistance by applying transgenic approaches , and eventually in the hope of cultivating new varities of crops with hoth highly - steady - yield and pest - resistance 本文尝试将透明颤菌血红蛋白( vhb )基因优化、人工合成并且分别和gfmcryia ( bt )基因、融合杀虫基因( bt - cpti )构建成双价基因植物高效表达载体，导入烟草，研究该基因对植物代谢水平和植物生长的影响，尝试通过基因工程育种技术增加转基因作物产量的同时，双可使其具有抗虫性，以期望培育出高产、稳产又具有抗虫效果的新品种。
By means of plant genetic engineering , foreign insects resistance gene can be transferred into plant cell . we cloned the cpti gene and transferred it into mustard by agrobacterium - mtdi & ted transformation method . and obtained the transgenic mustard plants . the main results are as follows : 1 . isolation of cpti gene total rna was isolated from cowpea seedss cotyledons and leaves . the cpti gene fragment was amplified by rt - pcr using sequences of its two sides as primers 本实验是利用植物基因工程获得抗虫的转基因芥菜植株，结果如下： 1豇豆胰蛋白酶抑制剂基因的分离分别提取豇豆种子、子叶及叶片的总rna ，逆转录成cdna 。以豇豆胰蛋白酶抑制剂基因两端的序列为引物，用rt - pcr的热启动方法从上述cdna中扩增出目的基因片断。
3 . the sequencing and analysis of cpti gene the sequencing of the single recombinant bacterium containg the inserted cpti gene fragment was carried out by daliang bio - engineering co . ltd . the result showed the fragment has 326bp , encoding 108 amino acid , the nucleotide acid homology was 99 % , the ammo acid homology was 79 % , comparing to cpti gene isloated by other researcher before 克隆片断的序列测定与分析挑选有插入片断的重组子单菌落，委托大连宝生物工程公司测序。结果表明：所克隆的目的片断为326bp ，编码108个氨基酸。经网上同源性比较，核苷酸的同源性为99 ，所推导的氨基酸的同源性为80 。
T . identification of charactrization of transgenic mustard plants the putative transformant regeneration plants were assayed by pcr and pcr - southern blot analysis . both analysis the target bands were observed . so the integration of the cpti gene into mustard genome dna was confirmed . the result of insect - resistance showed that the transgenic plants are more resistant than non - transgenic plants 转基因芥菜植株的鉴定取再生苗的叶片提取dna ，进行pcr扩增和pcrsouthernblot分析，转化再生植株大部分呈阳性，而非转化的再生植株均为阴性，证明cpt基因已存在于芥菜基因组中。
