The occurance of powdery mildew , rust , maruca testulalis geye of cowpea were studied after cowpea had been handled by different kinds of foliar fertilization , and the indexes such as ppo and yields , were measured . . the results showd that , using fertilizers reasonably could raise resistance of plants to pest , increase yields of crops and guarantee quality of products 摘要研究了经不同叶面肥处理后，豇豆白粉病、锈病、豆野螟的发生情况，测定了多酚氧化酶( ppo ) 、产量指标，结果表明，合理施肥能够提高植物对有害生物的抗性，增加产量，保证品质。
The cowpea trypsin inhibitor ( cptt ) gene is testified as a broad spectrum insect - resistant gene at present and its application in insect - resistant botanic transgenic engineering only after s / gene . the cpti transgenic plant developed rapidly for it ' s broad spectrum insect - resistant character and the target insects are uneasy tolerance to it 豇豆胰蛋白酶抑制剂( cpti )基因是目前在植物抗虫基因工程中应用仅次于bt基因的广谱性抗虫基因。鉴于它抗害虫的广谱性和靶标昆虫不易对其产生耐受性的优点，转cpti基因植物得到了迅速的发展。
By means of plant genetic engineering , foreign insects resistance gene can be transferred into plant cell . we cloned the cpti gene and transferred it into mustard by agrobacterium - mtdi & ted transformation method . and obtained the transgenic mustard plants . the main results are as follows : 1 . isolation of cpti gene total rna was isolated from cowpea seedss cotyledons and leaves . the cpti gene fragment was amplified by rt - pcr using sequences of its two sides as primers 本实验是利用植物基因工程获得抗虫的转基因芥菜植株，结果如下： 1豇豆胰蛋白酶抑制剂基因的分离分别提取豇豆种子、子叶及叶片的总rna ，逆转录成cdna 。以豇豆胰蛋白酶抑制剂基因两端的序列为引物，用rt - pcr的热启动方法从上述cdna中扩增出目的基因片断。
